اثر تنش خشکی بر ویژگی‌های مورفوفیزیولوژیکی و بیوشیمیایی نهال‌های ارغوان (.Cercis siliquastrum L)

نوع مقاله : علمی- پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه جنگل‌داری، دانشکده کشاورزی و منابع طبیعی، دانشگاه لرستان، خرمآباد، ایران

2 نویسنده مسئول، دانشیار، گروه جنگل‌داری، دانشکده کشاورزی و منابع طبیعی، دانشگاه لرستان، خرمآباد، ایران

3 دانش‌آموخته دکتری جنگل‌داری، دانشکده کشاورزی و منابع طبیعی، دانشگاه لرستان، خرمآباد، ایران

چکیده

ارغوان (Cercis siliquastrum L.) به‌عنوان یکی از گونه‌های مهم در ایجاد فضای سبز و جنگل‌کاری محسوب می‌شود. باتوجه‌به ارزش بوم‌شناختی و اقتصادی این گونه، آگاهی از نیاز آبی و مقاومت آن در برابر خشکی، ضروری است. پژوهش پیش‌رو با هدف بررسی تأثیر تنش خشکی بر ویژگی‌های مورفوفیزیولوژیکی و بیوشیمیایی نهال ارغوان در قالب طرح کامل تصادفی با چهار سطح تنش خشکی شامل ظرفیت زراعی 100 (شاهد)، 75 (تنش ملایم)، 50 (تنش متوسط) و 25 درصد (تنش شدید) در شرایط گلخانه‌ای در دانشکده کشاورزی و منابع طبیعی دانشگاه لرستان انجام شد. براساس نتایج به‌دست‌آمده، تنش خشکی شدید به‌طور معنی‌داری سبب کاهش در ویژگی‌های ارتفاع نهال، سطح برگ و محتوای نسبی آب برگ به‌ترتیب به‌مقدار 3/63، 7/77 و 6/62 درصد و افزایش در طول ریشه، نشت الکترولیت و غلظت مالون‌دی‌آلدئید به‌ترتیب به‌مقدار 1/42، 6/63 و 5/334 درصد در مقایسه با گیاهان شاهد شد. با تشدید تنش خشکی تا 25 درصد ظرفیت زراعی، فعالیت آنزیم‌های کاتالاز، پراکسیداز و آسکوربات‌پراکسیداز به‌ترتیب 862، 9/553 و 6/349 درصد افزایش یافت. این سطح از تنش سبب کاهش 55 درصدی در زنده‌مانی نهال‌ها شد. باتوجه‌به ارزیابی ویژگی‌های مورد بررسی می‌توان گفت که ارغوان، تنش خشکی تا 75 درصد ظرفیت زراعی را بدون آسیب تحمل می‌‌کند، اما کاهش آب تا حدآستانه 25 درصد ظرفیت زراعی سبب اختلال شدید در رشد و عملکرد آن می‌‌شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of drought stress on morphophysiological and biochemical traits of purple (Cercis siliquastrum L.) seedlings

نویسندگان [English]

  • Z. Saeidi abueshaghi 1
  • B. Pilehvar 2
  • S.V. Sayedena 3
1 M.Sc. Student, Department of Forestry, Faculty of Agriculture and Natural Resources, Lorestan University, Khorramabad, Iran
2 *- Corresponding author, Associate Prof., Department of Forestry, Faculty of Agriculture and Natural Resources, Lorestan University, Khorramabad, Iran
3 Ph.D. of Forestry, Faculty of Agriculture and Natural Resources, Lorestan University, Khorramabad, Iran
چکیده [English]

Purple (Cercis siliquastrum L.) is one of the most important species in landscaping and afforestation. Due to both ecological and economical values, knowledge on the water needs and resistance of purple to different levels of drought stress is essential. Therefore, an experiment in a completely randomized design with four levels of drought stress including field capacity 100% (control), 75% (mild stress), 50% (medium stress) and 25% (severe stress) was performed in greenhouse conditions to study the effect of drought stress on morpho-physiological and biochemical traits of purple seedlings. Results showed that severe drought stress decreased seedling height, leaf area, relative leaf water content (63.3, 77.7 and 62.6%, respectively) and increased electrolyte leakage and malondialdehyde concentration (63.6 and 334.5%, respectively) compared with plants in the control treatment. Drought stress level of 25% of field capacity increased root length by 42.1%. In addition, activity of catalase, peroxidase and ascorbate peroxidase enzymes (862, 553.9 and 349.6%, respectively) increased with increasing drought. The results also showed that only the level of drought stress of 25% of field capacity reduced the survival of seedlings by 55%. Therefore, this species was concluded to be able to withstand water stress up to 75% of field capacity without causing damage, while its growth and yield will be severely affected if the amount of water is reduced to the threshold of 25% of field capacity.

کلیدواژه‌ها [English]

  • Catalase
  • electrolyte leakage
  • peroxidase
  • relative leaf water content
  • seedling height

مراجع

- Alizadeh, A., 2000. Soil, Water, Plant Relationship. Imam Reza University Press, Mashhad, 356 (In Persian).
- Amini, Z., Moalemi, N.A. and Saadati, S., 2014. Effects of water deficit on proline content and activity of antioxidant enzymes among three olive (Olea europaea L.) cultivars. Journal of Plant Research (Iranian Journal of Biology), 27(2): 156-167 (In Persian).
- Asada, K., 1994. Mechanisms for scavenging reactive molecules generated in chloroplasts under light stress: 129-142. In: Baker, N.R. and Bowyer, J.R. (Eds.). Photoinhibition of Photosynthesis: from Molecular Mechanisms to the Field. Bios Scientific Publishers, Oxford, 471p.
- Asgharpour, E., Azadfar, D. and Saeedi, Z., 2017. Evaluation of Acer cappadocicum Gled seedlings to drought stress. Journal of Plant Research (Iranian Journal of Biology), 30(1): 1-11 (In Persian).
- Boot, R., Raynal, D.J. and Grime, J.P., 1986. A comparative study of the influence of drought stress on flowering in Urtica dioica and U. urens. Journal of Ecology, 74(2): 485-495.
- Chance, B. and Maehly, A.C., 1955. Assay of catalases and peroxidases: 764-775. In: Colowick, S.P. and Kaplan, N.O. (Eds.). Methods in Enzymology, Volume 2. Academic Press, New York, 987p.
- De Herralde, F., Biel, C., Savé, R., Morales, M.A., Torrecillas, A., Alarcón, J.J. and Sánchez-Blanco, M.J., 1998. Effect of water and salt stresses on the growth, gas exchange and water relations in Argyranthemum coronopifolium plants. Plant Science, 139(1): 9-17.
- Dichio, B., Romano, M., Nuzzo, V. and Xiloyannis, C., 2002. Soil water availability and relationship between canopy and roots in young olive trees (cv Coratina). Acta Horticulture, 586: 255-258.
- Djanaguiraman, M., Devi, D.D., Shanker, A.K., Annie Sheeba, J. and Bangarusamy, U., 2005. Selenium – an antioxidative protectant in soybean during senescence. Plant and Soil, 272(1): 77-86.
- Fang, Y. and Xiong, L., 2015. General mechanisms of drought response and their application in drought resistance improvement in plants. Cellular and Molecular Life Sciences, 72(4): 673-689.
- Fu, J., Fry, J. and Huang, B., 2004. Minimum water requirements of four turfgrasses in the transition zone. HortScience, 39(7): 1740-1744.
- Hasanuzzaman, M. and Fujita, M., 2011. Selenium pretreatment upregulates the antioxidant defense and methylglyoxal detoxification system and confers enhanced tolerance to drought stress in rapeseed seedling. Biological Trace Element Research, 143(3): 1758-1776.
- Hashempour, F., 2008. Investigation of the effect of drought stress on nine Eucalyptus species. M.Sc. thesis, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran, 410p (In Persian).
- Heidari, N., Poryousef, M. and Tavakoli, A., 2015. Effects of drought stress on photosynthesis, its parameters and relative water content of anise (Pimpinella anisum L.). Journal of Plant Research (Iranian Journal of Biology), 27(5): 829-839 (In Persian).
- Jaleel, C.A., Manivannan, P., Wahid, A., Farooq, M., Al-Juburi, H.J., Somasundaram, R. and Panneerselvam, R., 2009. Drought stress in plants: a review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology, 11(1): 100-105.
- Kamali, M., Kharazi, S.M., Selahvarzi, Y. and Tehranifar, A., 2012. Effect of salicylic acid on growth and some morphophysiological characteristics of Gomphrena (Gomphrena globosa L.) under salinity stress. Journal of Horticultural Science, 26(1): 104-112 (In Persian).
- Khan, A.S., Allah, S.U. and Sadique, S., 2010. Genetic variability and correlation among seedling traits of wheat (Triticum aestivum) under water stress. International Journal of Agriculture and Biology, 12(2): 247-250.
- Lei, Y., Yin, C. and Li, C., 2006. Differences in some morphological, physiological, and biochemical responses to drought stress in two contrasting populations of Populus przewalskii. Physiologia Plantarum, 127(2): 182-191.
- Li, C., Berninger, F., Koskela, J. and Sonninen, E. 2000. Drought responses of Eucalyptus microtheca provenances depend on seasonality of rainfall in their place of origin. Australian Journal of Plant Physiology, 27(3): 231-238.
- Lutts, S., Kinet, J.M. and Bouharmont, J., 1996. NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany, 78(3): 389-398.
- MacAdam, J.W., Nelson, C.J. and Sharp, R.E., 1992. Peroxidase Activity in the leaf elongation zone of tall fescue: I. Spatial distribution of ionically bound peroxidase activity in genotypes differing in length of the elongation zone. Plant Physiology, 99(3): 872-878.
- Mittler, R., Vanderauwera, S., Gollery, M. and Van Breusegem, F., 2004. Reactive oxygen gene network of plants. Trends in Plant Science, 9(10): 490-498.
- Nakano, Y. and Asada, K., 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22(5): 867-880.
- Nourozi Haroni, N., Tabari Kouchaksaraei, M. and Sadati, S.E., 2017. Response of growth indices of Judas tree seedling to different irrigation periods. Iranian Journal of Forest, 8(4): 419-430 (In Persian).
- Ranjbarfordoei, A., Samson, R., Van Damme, P. and Lemeur, R., 2000. Effects of drought stress induced by polyethylene glycol on pigment content and photosynthetic gas exchange of Pistacia khinjuk and P. mutica. Photosynthetica, 38(3): 443-447.
- Rao, P.B., Kaur, A. and Tewari, A., 2008. Drought resistance in seedlings of five important tree species in Tarai region of Uttarakhand. Tropical Ecology, 49(1): 43-52.
- Ritchie, S.W., Nguyen, H.T. and Holaday, A.S., 1990. Leaf water content and gas‐exchange parameters of two wheat genotypes differing in drought resistance. Crop science, 30(1): 105-111.
- Smirnoff, N., 1993. Tansley Review No. 52. The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist, 125(1): 27-58.
- Sternberg, P., 2012. Physiological and morphological basis for differences in growth, water use and drought resistance among Cercis L. taxa. Ph.D. thesis, Department of Horticulture and Crop Science, The Ohio State University, Columbus, Ohio, 170p.
- Wang, F., Zeng, B., Sun, Z. and Zhu, C., 2009. Relationship between proline and Hg2+-induced oxidative stress in a tolerant rice mutant. Archives of Environmental Contamination and Toxicology, 56(4): 723-731.
- Yin, C., Peng, Y., Zang, R., Zhu, Y. and Li, C., 2005. Adaptive responses of Populus kangdingensis to drought stress. Physiologia Plantarum, 123(4): 445-451.
- Zahreddine, H.G., Struve, D.K. and Talhouk, S.N., 2007. Growth and nutrient partitioning of containerized Cercis siliquastrum L. under two fertilizer regimes. Sciatica Horticulturae, 112(1): 80-88.
 

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