تأثیر نانوپرایمینگ بذر با نانولوله کربن چندجداره بر مؤلفه‌های جوانه‌زنی بذر و رشد اولیه گیاهچه‌های بارانک لرستانی (Sorbus luristanica)

نوع مقاله : علمی- پژوهشی

نویسندگان

1 دانشجوی دکتری جنگل‌شناسی و اکولوژی جنگل، دانشکده کشاورزی و منابع طبیعی دانشگاه لرستان، خرم‌آباد، ایران

2 دانشیار، گروه جنگل‌داری، دانشکده کشاورزی و منابع طبیعی دانشگاه لرستان، خرم‌آباد، ایران

3 استادیار، گروه جنگل‌داری، دانشکده کشاورزی و منابع طبیعی دانشگاه لرستان، خرم‌آباد، ایران

4 استادیار پژوهشی، بخش تحقیقات منابع طبیعی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان فارس، سازمان تحقیقات، آموزش و ترویج کشاورزی، شیراز، ایران

5 دانشیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی دانشگاه لرستان، خرم‌آباد، ایران

چکیده

در رابطه با شکست خواب بذر بارانک لرستانی (Sorbus luristanica) به‌عنوان یک گونه اندمیک هیچ گونه اطلاعاتی وجود ندارد. در این پژوهش از پتانسیل قابل توجه نانولوله‌­های کربنی چندجداره به‌منظور رفع خواب و ارتقاء جوانه‌­زنی این گونه استفاده شد. پیش از لایه‌گذاری گرم (دو هفته) و سرد (سه تا چهار ماه)، به‌عنوان تیمار پیشنهادی در این جنس، بذرها به مدت 24 ساعت با غلظت­‌های صفر، 75، 150، 250، 350 و 500 میلی­‌گرم در لیتر پرایم شدند. نتایج نشان داد که بذرهای این گونه حداقل به سه ماه لایه‌گذاری در سرما احتیاج دارد. پس از سپری شدن این مدت، بذرها در داخل پتری­دیش کشت و به ژرمیناتور انتقال یافتند. شمارش روزانه جوانه‌­زنی در یک دوره 22 روزه انجام و در پایان، درصد، سرعت و میانگین زمان جوانه‌­زنی محاسبه شد. همچنین، به‌منظور درک بیشتر تأثیر این نانومواد، رشد متعاقب گیاهچه­‌های رشدیافته از این بذرها نیز مورد مطالعه قرار گرفت. نتایج نشان داد که تیمار 350 میلی‌گرم در لیتر سبب ارتقاء تمام صفات جوانه‌­زنی در این گونه شد. مطالعات میکروسکوپیک حاکی از تحلیل دیواره آندوکارپ بذر به‌واسطه نانوپرایمینگ و افزایش نفوذ بیشتر رطوبت و اکسیژن به داخل بذر بود. افزایش رشد طولی و زی‌­توده ریشه بارزترین تأثیر نانوپرایمینگ بذر بود. به‌نظر می­رسد که تأمین رطوبت بیشتر به داخل گیاه یکی از سازکاروهای عملکردی این نانولوله کربن‌­های چند­جداره باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of Seed Nano-priming with Multiwall Carbon Nanotubes (MWCNT) on seed germination and seedlings growth parameters of mountain ash (Sorbus luristanica Bornm.)

نویسندگان [English]

  • Seyyed Vahid Sayedena 1
  • Babak Pilevar 2
  • Kambiz Abrari-Vajari 3
  • Mehrdad Zarafshar 4
  • Hamid Reza Eisvand 5
1 Ph.D. Student of Silviculture and Forest Ecology, Faculty of Agriculture and Natural Resources, Lorestan University, Khorram Abad, Iran
2 Associate Prof., Department of Forestry, Faculty of Agriculture and Natural Resources, Lorestan University, Khoram Abad, Iran
3 Assistant Prof., Department of Forestry, Faculty of Agriculture and Natural Resources, Lorestan University, Khoram Abad, Iran
4 Assistant Prof., Research Division of Natural Resources, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran
5 Associate Prof., Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, Lorestan University, Khoram Abad, Iran
چکیده [English]

A comprehensive study on breaking dormancy of mountain ash(Sorbus luristanica Bornm.) endemic species is still lacking. Here, the high efficient potentials of multiwall carbon nanotubes were used to break seed dormancy and improve seed germination in mountain ash. First, the seeds were primed with different concentrations of the nano-material including 0, 75, 150, 250, 350 and 500 mg l-1 during 24 hours. Then warm (two weeks) and cold (three or four months) stratifications were performed. The results showed that the seeds require cold stratification for at least 3 months. After the cold stratification period, the seeds were transferred to petri dishes in germinator. Daily seed germinations were recorded during 22 days. Eventually seed germination parameters such as seed germination percent, germination speed as well as mean germination time were calculated and compared. To understand the effects of nano-materials on the seedlings from different treatments, the subsequent growth of seeds was studied. The results revealed that all germination parameters were improved by 350 mg l-1 treatment. The microscopic seed observations showed that the nano-priming treatments led to seed endocarp abrasion and increased oxygen and moisture infiltration into seeds. Increasing root height growth was associated with the most considerable effects of treatments. Conclusively, it seems that increase in seedling moisture is one of the functional mechanisms of the MWCNT.

کلیدواژه‌ها [English]

  • biomass
  • germination speed
  • germinator
  • Seed dormancy
  • seed endocarp
  • Stratification

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