مقایسه ویژگی‌های ریخت‌شناسی و ریزریخت‌شناسی برگ در دورگ‌های بین‌گونه‌ای صنوبر

نوع مقاله : علمی- پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، دانشگاه پیام نور اصفهان، اصفهان، ایران

2 دانشیار پژوهش، مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

3 استاد پژوهش، مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

4 استادیار، دانشکده منابع طبیعی و محیط زیست، دانشگاه پیام نور، تهران، ایران

چکیده

 
به‌منظور انجام پژوهش پیش‌رو، تلاقی‌های متعددی بین پایه‌های پده (Populus euphratica Oliv.) و کبوده (Populus alba L.) در سال‌های 1390 تا 1391 انجام شد. از کشت جنین نارس دورگ‌ها در محیط کشت MS برای تولید نهال دورگ‌ها استفاده شد. چندین ویژگی‌ ریخت‌شناسی و ریز‌ریخت‌شناسی در تعداد زیادی از ژنوتیپ‌‌های دورگ جدید شش خانواده متفاوت پس از دو سال رویش در مزرعه، در سه تکرار اندازه‌گیری و تجزیه و تحلیل شدند تا با ارزیابی قابلیت‌‌هایی مانند تعداد، طول و زاویه شاخه‌ها، ابعاد برگ و قطر یقه در دورگ‌های به‎دست‌آمده و شناسایی دورگ‌های برتر، تفاوت بین دورگ‌ها با والدین مادری پده یا کبوده نیز ارزیابی شود. خانواده‎ها در بیشتر صفات اختلاف معنی‎داری داشتند. در برخی صفات، تفاوت در درون تلاقی‎ها معنی‎دار شد و نتاج به‎دست‌آمده از دو گروه تلاقی P. euphratica×P. alba و P. alba×P. euphraticaدر دو دسته متمایز قرار گرفتند که نشان‎دهنده اثرات مادری بود. دامنه صفات در نتاج به‎دست‌آمده بسیار گسترده بود. به‎عنوان نمونه در بین تک‎پایه‎های نتاج، ارتفاع نهال‎ها بین 24 تا 424 سانتی‎متر متغیر بود. بارزترین اختلاف‎ها در ویژگی‎های روزنه‎ بود. دورگ‎ها با والد مادری پده و کبوده، از نظر برخی از صفات رفتارهای بسیار متفاوتی نشان دادند. در تلاقی‎هایی که پده به‎عنوان گونه مادری استفاده شده بود، میانگین کل تعداد روزنه در سطح زبرین برگ 123 عدد در میلی‌متر مربع بود، درحالی‌که در تلاقی‎هایی که کبوده به‎عنوان والد مادری استفاده شده بود، میانگین کل این صفت برابر با 8/4 عدد بود. این تنوع می‎تواند در انتخاب ژنوتیپ‎های برتر و معرفی کلن‎های جدید صنوبر استفاده شود.
 

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of poplar inter-specific progenies based on their morphologic and micro-morphologic traits

نویسندگان [English]

  • Fatemeh Tavousi Rad 1
  • Abbas Ghamari Zare 2
  • Hosein Mirzaie-Nodoushan 3
  • Maryam Usefifard 4
1 M.Sc. Student, Isfahan Payame Noor University, Isfahan, Iran
2 Associate Prof., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
3 Prof., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
4 Assistant Prof., Faculty of Environment and Natural Resources, Payame Noor University, Tehran, Iran
چکیده [English]

In this study, numerous crosses were initially performed between male and female parents of two poplar species, P. euphratica Oliv. and P. alba L.. Aseptic immature hybrid embryos were cultured on MS medium to produce hybrid seedlings. Several morphologic and micro-morphologic characteristics of a great number of three year-old seedlings of new genotypes based on six half-sib families were recorded with three replications, with the aim to assess possible potentials of the hybrids between the two species. An additional goal was to compare the hybrids of P. alba as either male or female parents. Analysis of variance revealed significant differences between the families. Two crossings, P. alba×P.euphratica and P. euphratica×P. alba, formed two distinct groups for some of the characteristics which partially implied maternal effects of inheritance on the traits. Range of the attributes was significantly wide, as for example the progenies varied between 24 and 424 cm of height. The most noticeable difference was observed on stomata characters. The hybrids with the two species as their female parent showed significantly different behaviors on several traits. The total average of leaf upper side stomata number was 123 for the crosses with P. euphratica as female parent, whereas the value was 4.8 in the crosses with P. alba as female parent. The variation might be beneficial is selecting superior genotypes to introduce new poplar varieties.
 

کلیدواژه‌ها [English]

  • Inter-specific hybrids
  • micro-morphologic traits
  • Populus alba
  • Populus euphratica
  • stomata

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