تأثیر تلقیح باکتریهای محلول کننده فسفات بر دانه‌رستهای افرا پلت (Acer velutinum)

نوع مقاله : علمی- پژوهشی

نویسندگان

اعضای هیات علمی موسسه تحقیقات جنگلها و مراتع کشور

چکیده

میکروارگانیسم‌های خاک از اجزای مهم اکوسیستم‌های جنگلی هستند. اصطلاح PGPR برای باکتریهای غیر‌همزیست خاک که توانایی کلنیزه شدن بر روی ریشه گیاهان و تحریک رشد را دارند بکار می‌رود. هدف این بررسی، تعیین اثرات تلقیح باکتریهای محلول‌کنندة فسفات بر روی دانه‌رستهای افرا پلت (Acervelutinum) بود. باکتریهای جدا شده از خاکهای جنگل ”واز“ (شاملPsudomonas, Pasturella, Actinobacillus, Enterobacter, Alcaligenese) در محیط کشت مایع Seperb و در دمایC ° 25 کشت داده شدند. سلولهای باکتریایی با سانتریفوژ جدا و با استفاده از محلول بافر فسفات نمکی (2/7pH =) سوسپانسیونی از باکتریها به غلظت   cfu/ ml 108× 3 تهیه شد. بذرهای افراپلت در گلدانهای پلاستیکی کاشته و با  1 میلی‌لیتر از سوسپانسیون باکتری تلقیح شدند. به بذرهای کنترل محلول بافر فسفات نمکی اضافه شد. پس از هفت ماه دانه‌رستها برداشت و درصد وزن خشک برگها و ریشه‌ها ، طول شاخه و ریشه، فسفر در دسترس در خاک و گیاه، عناصر آهن، پتاسیم و منیزیوم اندازه‌گیری شدند. دانه‌رستهای تلقیح‌شده دارای سیستم ریشه‌ای وسیعتری در مقایسه با دانه‌رستهای کنترل بودند.تأثیر باکتریهای مختلف بر خصوصیات مختلف دانه‌رستها متفاوت بود. در مجموع نتایج نشاندهنده اثرات مثبت باکتریهای محلول کننده فسفات رشد دانه رستهای افرا پلت بود و به‌نظر می‌رسد که تلقیح مخلوطی از باکتریها در تحریک رشد تأثیر زیادی خواهد داشت.
 

کلیدواژه‌ها

عنوان مقاله [English]

Effect of posphate solubilizing bacteria inoculation on Maple (Acer velutinum) seedlings

نویسندگان [English]

  • Maryam Teimouri
  • Soodabeh Korori
  • Mostafa Khoshnevis
  • Mohammad Matinizadeh
  • Mohsen Kalagari
Member of Scientific Board . Research Institute of Forests and Rangelands
چکیده [English]

     Soil microorganisms are important components of forest ecosystems. The term plant growth promoting rhizobacteria (PGPR) is used to describe non-symbiotic soil bacteria, which have potential to colonize plant root and stimulate growth. The aim of study was assessment the effects of Acer velutinum seeds inoculation with PSBs. Five isolated bacterial strains (including Pasturella, Acintobacillus, Pseudomonas, Alcaligense and Enerobacter) were cultured separately in liquid Seperb medium at 25 ºC. Bacterial cells were isolated by centrifuge and resuspended in phosphate buffer saline (pH= 7.2) to a density of 3×108  cfu per ml. Seeds of Acer velutinum were sowed in plastic pots and inoculated with bacterial suspension. Control seeds received sterile phosphate buffer saline. After seven months, seedlings were harvested. Shoot height, root length and their dry matter, available phosphorous in soil and seedlings were determined by standard methods. Results indicated the positive effect of PSBs on seedling emergence of Acer velutinum. Inoculated seedlings had more developed root system than those of controls. In conclusion, results indicated the stimulatory effect of PSBs on seedling emergence and their growth. According to results, it seemed inoculation of a mixed inoculums will be more effective in stimulating of growth. 
Acer velutinum, PSBs, inoculation, seedling.

کلیدواژه‌ها [English]

  • Acer velutinum
  • PSBs
  • Inoculation
  • Seedling

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