اثر تیمارهای ایندول بوتیریک اسید، باکتریایی و قارچی بر ریشه‌زایی قلمه‌های زالزالک ایروانی (Crataegus pseudoheterophylla Pojark.)

نوع مقاله: علمی- پژوهشی

نویسندگان

1 دکترای جنگل‌داری، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس

2 استاد، گروه جنگل‌داری، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس

3 مربی پژوهش، بخش تحقیقات منابع طبیعی، مرکز تحقیقات کشاورزی و منابع طبیعی مرکزی

چکیده

پژوهش پیش‌رو به‌منظور یافتن شیوه‌نامه مناسب برای ریشه‌زایی و تولید نهال زالزالک ایروانی (Crataegus pseudoheterophylla Pojark.) از طریق قلمه چوب سخت با تیمارهای ایندول بوتیریک اسید (IBA)، باکتریایی و قارچی با دو منشأ متفاوت جست و نهال انجام شد. قلمه‌ها در دو آزمایش جداگانه در قالب طرح کامل تصادفی با 11 تیمار و سه تکرار 10 تایی در بستر شاسی گرم کشت شدند. تیمارها شامل قرار دادن انتهای قلمه در 5/3 درصد وزنی حجمی آب اکسیژنه (H2O2) و سپس کاربرد IBA با غلظت‌های صفر (شاهد)، یک، دو، سه، پنج، هشت و 10 ‌گرم در لیتر، قرار دادن انتهای قلمه در محلول چهار ‌گرم در لیتر IBA و سپس قرار دادن در سوسپانسیون باکتریایی (Agrobacterium rhizogenes A13، Pseudomonas fluorescens 169 وBacillus subtilis FzB24) و مایه تلقیح قارچی‌  Glomus intraradicesبود. در آزمایش اول قلمه‌هایی با منشأ جست در بستر شاسی کشت شدند و پس از گذشت سه ماه در شرایط عرصه بازکاشت نهال‌ها انجام شد و خصوصیات ریشه‌زایی نهال‌ها پس از گذشت یک فصل رویشی ارزیابی شد. در آزمایش دوم از نهال‌های تولیدشده از آزمایش اول قلمه تهیه شد و آزمایش در شرایط گلخانه و عرصه و با تیمارهایی همانند آزمایش اول انجام شد. نتایج نشان داد که در آزمایش اول بیشترین درصد ریشه‌زایی در ترکیب H2O2 با غلظت سه ‌گرم در لیتر IBA و بیشترین مشخصه‌های سطح ریشه، سطح ویژه برگ، وزن خشک نهال، ارتفاع و کلروفیل مربوط به تیمار ترکیبی IBA با سوسپانسیون باکتریایی A. rhizogenes بود. در آزمایش دوم، بیشترین درصد ریشه‌زایی در تیمارهای ترکیب H2O2 باغلظت سه ‌گرم در لیتر و پنج ‌گرم در لیتر IBA و تیمار ترکیبی IBA با سوسپانسیون باکتریایی A. rhizogenes مشاهده شد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of IBA, bacterial and mycorrhizal treatments on the rooting of Crataegus pseudohetrophylla Pojark. cuttings

نویسندگان [English]

  • Fateme Ahmadloo 1
  • Masoud Tabari Kouchaksaraei 2
  • Gholam Reza Goodarzi 3
1 Ph.D. Forestry, Department of Forestry, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University
2 Prof., Department of Forestry, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University
3 Senior Research Expert, Research Division of Natural Recourses, Markazi Agricultural and Natural Resources Research Center, AREEO
چکیده [English]

This study was carried out to determine appropriate methods for rooting and seedling production of Crataegus pseudohetrophylla Pojark. from hardwood cuttings by IBA, bacterial and mycorrhizal treatments and two different sources of sprout and seedling. For this purpose, cuttings were sown in two separate experiments in a Complete Randomized Design (CRD) with 11 treatments and 3 replications of 10 cuttings in greenhouse benches. Treatments contained dipping the basal end of cuttings in H2O2 (3.5% w/v) followed by the application of IBA at concentrations (0, 1, 2, 3, 5, 8, and 10 g l-1) or dipping in 4 g l-1 IBA and then application of bacterial suspension of Agrobacterium rhizogenes A13, Pseudomonas fluorescens 169, Bacillus subtilis FzB24, and mycorrhizal inoculums of Glomus intraradices. In the first experiment, cuttings from sprout source were sown in greenhouse benches. The seedlings were transplanted to the open field condition after three months, and were assessed for rooting characteristics after a season of growth. In the second experiment, cuttings were prepared from seedlings produced from the first experiment, and the experiment was conducted in greenhouse and open field conditions and with similar treatments to those applied in the first experiments. Results showed that in the first experiment the highest rooting percentage was obtained in the combination of H2O2 with 3 g l-1IBA, and the highest root area, specific leaf area, total seedling dry weight, height, and total chlorophyll contents were obtained when a combination of IBA with bacterial suspension of A. rhizogenes was applied. In the second experiment, the highest rooting percentage was observed for the combination of H2O2 with 3 and 5g l-1IBA, as well as for the combination of IBA with bacterial suspension of A. rhizogenes.

کلیدواژه‌ها [English]

  • Auxin
  • greenhouse benches
  • Bacterial inoculation
  • rooting
  • root area
  • source of cutting
- Aghakhani, S. and Metaji, A., 2010. The study of ecological and seriate structure of Markazi province jungles. Plant Ecophysiology (Arsanjan Branch), 1(3): 54-62 (In Persian).

- Ahemad, M. and Kibret, M., 2014. Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. Journal of King Saud University Science, 26(1): 1-20.

- Ahmadloo, F., Tabari, M., Azadi, P. and Hamidi, A., 2014. Effect of plant growth promoting rhizobacteria (PGPRs) and stratification on germination traits of Crataegus pseudoheterophylla Pojark seeds. Journal of Scientia Horticulturae, 172(9): 61-67.

- Alizadeh, A., 2005. Water, Soil and Plant Relationship. Emam Reza Press, Mashhad, 470p (In Persian).

- Azizi, M., Aghabozorgee, M., Farsee, M., Tehranifar, A., Zolalla, J. and Ghabooli, M., 2007. Study on the root adventitious root formation in some horticultural crops using Agrobacterium rhizogenes. Agricultural Sciences and Technology (Special Issue in Horticulture Science), 21(2): 79-87 (In Persian).

- Bassil, N.V., Proebsting, W.M., Moore, L.W. and Lightfoot, D.A., 1991. Propagation of hazelnut stem cuttings using Agrobacterium rhizogenes. Horticultural Science, 26(8): 1058-1060.

- Bates, R.M. and Niemiera, A.X., 1996. A comparison of morphological features affecting water loss in Norway maple and Washington hawthorn stems. Journal of Environmental Horticulture, 14(2): 71-76.

- Caboni, E., Lauri, P., Tonelli, M., Falasca, G. and Damiano, C., 1996. Root induction by Agrobacterium rhizogenes in walnut. Plant Science, 118: 203-208.

- Dirr, M.A. and Heuser, C.W. Jr., 1987. The Reference Manual of Plant Propagation: From Seed to Tissue Culture. Varsity Press, Athens, Georgia, 239p.

- Dirr, M.A., 1998. Manual of Woody Landscape Plants, Their Identification, Ornamental Characteristics, Culture, Propagation and Uses. Stipes Publishing L.L.C., Athens, Georgia, 1187p.

- Ercan, A., Taskin, M., Turgut, K. and Yuce, S., 1999. Agrobacterium rhizogenes-mediated hairy root formation in some Rubia tinctorum L. populations grown in Turkey. Turkish Journal of Botany, 23(6): 373-377.

- Ercisli, S., Esitken, A. and Sahin, F., 2004. Exogenous IBA and inoculation with Agrobacterium rubi stimulate adventitious root formation on hardwood stem cuttings of two Rose genotypes. HortScience, 39(3): 533-534.

- Esitken, A., Ercisli, S., Sevik, I. and Sahin, F., 2003. Effect of indole-3-butyric acid and different strains of Agrobacterium rubi on adventive root formation from softwood and semi-hardwood wild sour cherry cuttings. Turkish Journal of Agriculture & Forestry, 27: 37-42.

- Exadaktylou, E., Thomidis, T., Grout, B., Zakynthinos, G. and Tsipouridis, C., 2009. Methods to improve the rooting of hardwood cuttings of the ‘Gisela 5’ cherry rootstock. Horticulture Technology, 19(2): 254-259.

- Hartmann, H.T., Hudson, T., Kester, D.E., Dale, E.K., Davies, J.R.F.T. and Geneve, R.L., 2002. Plant Propagation: Principles and Practices. Prentice-Hall Press, London, 770p.

- Husen, A. and Pal, M., 2007. Metabolic changes during adventitious root primordium development  in Tectona grandis Linn. F. (teak) cuttings as affected by age of donor plants and auxin (IBA and NAA) treatment. New Forests, 33(3): 309-323.

- Indiragandhi, P., Anandham, R., Madhaiyan, M. and Sa, T.M., 2008. Characterization of plant growth promoting traits of bacteria isolated from larval guts of diamondback moth Plutella xylostella (Lepidoptera: Plutellidae). Current Microbiology, 56: 327-333.

- Jafari, M. and Bouzari, N., 2010. Effect of different times of collection and hormone concentrations on rooting of hard and semi-hard wood cuttings in gisela6 cherry rootstock, Seed and Plant Production Journal, 26(3): 343-357 (In Persian).

- Karakurt, H., Aslantas, R., Ozkan, G. and Guleryuz, M., 2009. Effects of indol–3-butyric acid (IBA), plant growth promoting rhizobacteria (PGPR) and carbohydrates onrooting of hardwood cutting of MM106 apple rootstock. African Journal of Agricultural Research, 4 (2): 060-064.

- Kebede, M., Hulten, H. and Balcha, G., 2013. Vegetative propagation of juvenile leafy stem cuttings of Prunus Africana (Hook. F.) Kalkm and Syzygium guineense (Willd.) DC. International Journal of Botany, 9(1): 30-36.

- Liao, W.B., Xiao, H.L. and Zhang, M.L., 2010. Effect of nitric oxide and hydrogen peroxide on adventitious root development from cuttings of ground-cover chrysanthemum and associated biochemical changes. Journal of Plant Growth Regulation, 29: 338-348.

- Lichtenthaler, H.K. and Wellburn, A.R., 1983. Determinations of total carotenoids and chlorophylls a and b in leaf extracts in different solvents. Biochemical Society Transactions, 11: 591-592.

- Mozaffarian, V., 2004. Trees and Shrubs of Iran. Published by Farhang-e Moaser, Tehran, 1120p (In Persian).

- Payne, J.A. and Krewer, G.W., 1990. Mayhaw: a new fruit crop for the South: 317-321. In: Janick, J. and Simon, J.E., (Eds.). Advances in New Crops. Timber Press, Portland, 321p.

- Qrunfleh, M.M., 1993. Studies on the hawthorn (Crataegus azarolus L.): III. A potential rootstock for ‘Golden Delicious’ apple and ‘Williams’ pear. The Journal of Horticultural Science and Biotechnology, 68: 983-988.

- Sadati, S.E., Tabari, M., Assareh, M.H., Heidari Sharifabad, H. and Fayaz, P., 2010. Effect of cutting source and planting depth on vegetative propagation of Populus caspica Bornm. Iranian Journal of Forest and Poplar Research, 18(4): 667-679 (In Persian).

- Sadeghi, H. and Rajab Nezhad, K., 2010. Evaluation of simultaneous application of boric acid, hydrogen proxide and tiamine accompanied with indole-3-butyric acid on rooting of olive cuttings cv. Rashid. Iranian Journal of Horticultural Sciences, 41(2): 173-178 (In Persian).

- Sarrou, E., Therios, I. and Dimassi-Theriou, K., 2014. Melatonin and other factors that promote rooting and sprouting of shoot cuttings in Punica granatum cv. Wonderful. Turkish Journal of Botany, 38(2): 293-301.

- Scagel, C.F., 2001. Cultivar specific effects of mycorrhizal fungi on the rooting of miniature rose cuttings. Journal of Environmental Horticulture, 19(1): 15-20.

- Scagel, C.F., 2004. Changes in cutting composition during early stages of adventitious rooting of miniature rose altered by inoculation with Arbuscular mycorrhizal fungi. Journal of the American Society for Horticultural Science, 129(5): 624-634.

- Sebastiani, L., Tognetti, R., Dipaolo, P. and Vitagliano, C., 2004. Hydrogen peroxide and indole-3-butyric acid effects on root induction and development in cuttings of Olea europaea L. cv. Frantoio and Gentilre di larino. Horticultural Science, 16(1): 7-12.

- Stenvall, N., Haapala, T. and Pulkkinen, P., 2004. Effect of genotype, age and treatment of stock plants on propagation of hybrid aspen (Populus tremula×Populus tremuloides) by root cuttings. Scandinavian Journal of Forest Research, 19: 303-311.

- Tamasloukht, M.B., Séjalon-Delmas, N., Kluever, A., Jaunean, A., Roux, C., Bécard, G. and Franken, P., 2003. Root factors induce mitochondrial-related gene expression and fungal respiration during the developmental switch from asymbiosis to presymbiosis in the Arbuscular mycorrhizal fungus Gigaspora rosea. Plant Physiology, 131(3): 1468-1478.

- Yeboah, J., Lowor, S.T. and Amoah, F.M., 2009. The rooting performance of shea (Vitellaria paradoxa Gaertn) stem cuttings as influenced by wood type, sucrose and rooting hormone. Scientific Research and Essay, 4(5): 521-525.

- Zhao, D., Reddy, K.R. Kakani, V.G. and Reddy, V.R., 2005. Nitrogen deficiency effects on plant growth, leaf photosynthesis and hyperspectral reflactant properties of sorghum. European Journal of Agronomy, 22(4): 391-403.