اثر سرمای دیررس شبانه بر اجزای فتوسیستم دو در سه کلن شالک (Populus nigra L.)

نوع مقاله : علمی- پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد جنگل‌داری، دانشکده کشاورزی، دانشگاه یاسوج

2 استادیار، گروه جنگل‌داری، دانشکده کشاورزی و پژوهشکده منابع طبیعی و محیط زیست، دانشگاه یاسوج

چکیده

ارزیابی عملکرد فتوسیستم دو به‌عنوان پرانرژی‌ترین فتوسیستم که قادر به شکستن آب و تأمین الکترون مورد نیاز مرحله نوری فتوسنتز است، از اهمیت ویژه‌ای برخوردار است. سرمای دیررس که به‌طور معمول در فصل رویش و در طول شب اتفاق می‌افتد، با آسیب رساندن به اندام‌های در حال رشد گیاه، موجب ضعف و حتی مرگ آنها می‌شود. در این پژوهش به‌منظور بررسی اثر سرمای دیررس شبانه بر اجزای عملکردی فتوسیستم دو و برخی از ویژگی‌های فیزیولوژیکی مرتبط با آن، نهال‌های سه کلن شالک (Populus nigra L.) به‌مدت سه شب در معرض حداقل دماهای شبانه 16، چهار، صفر و 20- درجه سانتی‌گراد قرار گرفتند. سپس نهال‌ها به‌مدت 14 روز در دمای بهینه به‌منظور بررسی توان احیا نگهداری شدند. داده‌ها با استفاده از مدل فاکتوریل با دو عامل کلن در سه سطح و حداقل دمای شبانه در چهار سطح به‌تفکیک برای مرحله تنش و احیا تجزیه و تحلیل شدند. نتایج نشان داد که عملکرد ماکزیمم و مؤثر فتوسیستم دو تحت تأثیر کارایی کمپلکس تجزیه‌کننده آب و فعالیت مخزن پلاستوکوئینون در کلن‌های مختلف روند کاهشی متفاوتی را نشان داد، به‌نحوی‌که کلن ایرانی حساس‌تر از کلن‌های دیگر بود. این تفاوت در ارتباط با محتوای قند محلول، پرولین، کلروفیل و نرخ نشت الکترولیت‌ها از غشای سیتوپلاسمی بررسی شد. همچنین روند بهبودی کلن‌ها در دوره احیا مقایسه شد.

کلیدواژه‌ها

عنوان مقاله [English]

Impacts of night late frost on photosystem II components of three black poplar (Populus nigra L.) clones

نویسندگان [English]

  • Ma'soumeh Hasanvand 1
  • Payam Fayyaz 2
1 M.Sc. Student Forestry, Faculty of Agriculture, Yasouj University
2 Assistant Prof., Department of Forestry, Faculty of Agriculture and Institute of Natural Resources and Environment, Yasouj University
چکیده [English]

It is of crucial importance to assess the performance of the photosystem II efficiency, as the most powerful system in light reactions of photosynthesis that is able to provide required electron of system by splitting water. Late frost occurred mostly in growing season and during night in early spring often results in weakness and even death of plants. Therefore, selecting tolerant varieties is one of the most efficient methods to deal with late frost. Due to high diversity and wide dispersal, poplars significantly contribute to the worldwide supply of cellulosic resources. In order to investigate the effect of night late frost on functional component of photosystem II and a number of related physiological traits, seedlings of three clones of black poplar (Populus nigra L.) were exposed to the minimum night temperature of 16, 4, 0 and –20 °C for three nights. To assess the revival potential, seedlings were further maintained in optimum temperature for 14 days. Data were analyzed using a factorial model with two factors of clone and minimum night temperature for each step of the stress and revival. Results revealed that maximum and quantum yield efficiency of photosystem II in different clones has different reduction patterns according to the efficiency of water splitting complex and plastoquinon pool (PQ). Thus, Iranian clone was concluded to be more sensitive than other clones. This difference was discussed in association with total soluble sugar, proline and chlorophyll concentration and electrolyte leakage rate of cytoplasmic membrane. In addition, the recovery process of the clones in revival period was discussed.

کلیدواژه‌ها [English]

  • Chilling
  • chlorophyll fluorescence
  • Freezing
  • plastoquinon pool activity
  • water splitting complex

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