ارزیابی روش کشت گلخانه‌ای مطالعه بانک بذر خاک در دو حالت با شستشو و بدون شستشو

نوع مقاله: علمی- پژوهشی

نویسندگان

1 کارشناس ارشد جنگل‌داری، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس

2 دانشیار، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس

3 استادیار، دانشکده منابع طبیعی و علوم دریایی، دانشگاه تربیت مدرس

چکیده

هدف از پژوهش پیش‌رو، ارزیابی نتایج بانک بذر خاک در جنگل جلگه‌ای دانشگاه تربیت مدرس با استفاده از دو روش با شستشو و بدون شستشو بود. برای این منظور، چهار قطعه‌نمونه 40×40 متر مربعی که هریک شامل چهار قاب فرعی 400 متر مربعی بودند، به‌عنوان توده معرف انتخاب شدند. نمونه‌برداری از پوشش گیاهی روزمینی در 16 قطعه‌نمونه 400 متر مربعی در دو زمان اسفند و خرداد انجام شد. مطالعه بانک بذر خاک نیز با استفاده از روش کشت گلخانه‌ای به‌مدت هفت ماه براساس 64 (2 روش × 4 قطعه‌نمونه × 4 تکرار × 2 عمق صفر تا پنج و پنج تا 10 سانتی‌متری) نمونه خاک 40×40 سانتی‌متر مربعی انجام شد. تجزیه و تحلیل آماری با استفاده از طرح قطعه‌نمونه‌های خردشده در مکان در قالب طرح بلوک کامل تصادفی انجام شد. نتایج نشان داد که اندازه و غنای گونه‌ای بانک بذر خاک در روش بدون شستشو (در عمق اول 63 گونه و 1872 بذر در هر متر مربع و در عمق دوم 46 گونه و 975 بذر در هر متر مربع) به‌طور معنی‌داری بیشتر از روش با شستشو (در عمق اول 45 گونه و 1137 بذر در هر متر مربع و در عمق دوم 33 گونه و 699 بذر در هر متر مربع) بود. نتایج همچنین نشان داد که در هر دو روش مطالعه، با افزایش عمق خاک از درجه تشابه گونه‌ای بانک بذر خاک و پوشش گیاهی متناظر کاسته می‌شود، اما در این رابطه نتایج این دو روش با یکدیگر اختلاف معنی‌داری را نشان نداد. به‌طور کلی نتایج پژوهش پیش‌رو نشان داد که در مطالعه بانک بذر خاک استفاده از روش کشت گلخانه‌ای بدون شستشو نسبت به روش با شستشو به‌دلیل ارایه نتایج بهتر در اولویت است.

کلیدواژه‌ها


عنوان مقاله [English]

Assessment of seedling emergence method in soil seed bank in two states of washing and non-washing

نویسندگان [English]

  • Fateme Akbarpour 1
  • Seyyed Gholam Ali Jalali 2
  • Omid Esmaeilzadeh 3
1 M.Sc. Forestry, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University
2 Associate Prof., Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University
3 Assistant Prof., Faculty of Natural Resources and Marine Science, Tarbiat Modares University
چکیده [English]

This study aimed to evaluate the results of soil seed bank in Tarbiat Modares Lowland Experimental Forest by using the two methods of washing and non-washing. Therefore, four main plots (40×40 m2)with each of them comprising 4 sub quadrats of 400 m2 were considered as representative stands. Aboveground vegetation was sampled by 16 plots of 400 m2 in two time steps of March and June. Seed bank flora was studied using seedling emergence method based on 64 soil samples (2 methods×4 main plots×4 replications×2 depths of 0-5 and 5-10 cm) which had been hammered by a hollow 400 cm2 metal frame. Statistical analysis was performed using split plot in space set within a randomized complete block experimental design. Results showed that both species richness and density of soil seed bank in non-washing method (63 species with 1872 seed density per m2 in upper layerand 46 species with 975 seed density in lower layer) were significantly higher than in washing method (45 species with 1137 seed density per m2 in upper layerand 33 species with 699 seed density in lower layer). Results also showed that similarity between soil seed bank with corresponding extant vegetation decreased with increasing soil depth. However, the two methods were not significantly different. We concluded that seedling emergence method without washing results in better soil seed bank assessment.

کلیدواژه‌ها [English]

  • Soil seed bank
  • similarity
  • lowland forest of Tarbiat Modares
  • soil washing
- Asadi, H., Hosseini, S.M., Esmailzadeh, O. and Baskin, C.C., 2012. Persistent soil seed banks in old growth Hyrcanian box tree (Buxus hyrcana) stands in Nothern Iran. Ecological Research, 27(1): 23-33.

- Asadi, M., Masoumi, A.A., Khatamsaz, M. and Mozaffarian, V., 1992-2002. Flora of Iran. Published by Research Institute of Forests and Rangelands, Tehran, Volume 1-38 (In Persian).

- Baskin, C.C. and Baskin, J.M., 1998. Germination ecology of seeds in the persistent seed bank: 133–180. In: Baskin, C.C. and Baskin, J.M., (Eds.). Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination. Academic Press, San Diego, CA.

- Bernhardt, K.G., Koch, M., Kropf, M., Ulbel, E. and Webhofer, J., 2008. Comparison of two methods characterising the seed bank of amphibious. Aquatic Botany, 88(2): 171-177.

- Bossuyt, B. and Honnay, O., 2008. Can the seed bank be used for ecological restoration? an overview of seed bank characteristics in European communities. Journal of Vegetation Science,19(6): 875-884.

- Bossuyt, B., Heyn, M. and Hermy, M., 2000. Concentrating samples estimates larger seed bank density of a forest soil. Functional Ecology, 14(6): 766-767.

- Brown, D., 1992. Estimating the composition of a forest seed bank: a comparison of the seed extraction and seedling emergence methods. Canadian Journal of Botany, 70(8): 1603–1612.

- Crocker, W., 1919. Buried weed seeds. Botanical Gazette, 67: 515-516.

- Esmailzadeh, O., Hosseini, S.M., Mesdaghi, M., Tabari, M., Mohammadi, J., 2010a. Can soil seed bank floristic data describe above ground vegetation plant communities?. Environmental Sciences, 7(2): 41-62 (In Persian).

- Esmailzadeh, O., Hosseini, S.M., Mesdaghi, M., Tabari, M., Mohammadi, J., 2010b. Persistent soil seed bank study of Darkola oriental beech (Fagus orientalis Lipsky) forest. Journal of Forest and Wood Products, 63(2): 117-135 (In Persian).

- Esmailzadeh, O., Hosseini, S.M., Tabari, K.M., Baskin, C.C. and Asadi, H., 2011. Persistent soil seed banks and floristic diversity in Fagus orientalis forest communities in the Hyrcanian vegetation region of Iran. Flora, 206(4): 365-372.

- Fenner, M. and Thompson, K., 2005. The Ecology of Seeds. Cambridge University Perss, Cambridge, 262p.

- Ghahraman, A., 1996-2000. Iran Colored Flora. Published by Research Institute of Forests and Rangelands, Tehran, Volume 1-22 (In Persian).

- Gonzalez, S.L. and Ghermandi, L., 2012. Comparison of methods to estimate soil seed banks: the role of seed size and mass. Community Ecology, 13(2): 238-242.

- Hammerstrom, K.K. and Kenworthy, W.J., 2003. A new method for estimation of Halophila decipiens Ostenfeld seed banks using density separation. Aquatic Botany, 76(1): 79-86.

- Ishikawa, M. and Tsuyuzaki, G.S., 2004. Methods of estimating seed banks with reference to long-term seed burial. Journal of Plant Research, 117(3): 245-248.

- Johnson, R.G. and Anderson, R.C., 1986. The seed bank of a tallgrass Prairiein Illinois. American Midland Naturalist, 115(1): 123-130.

- Malone, R., 1967. A rapid method for enumeration of viable seeds in soil. Weeds, 15(4): 381-382.

- Martinez-Duro, E., Luzuriaga, A.L., Ferrandis, P., Escudero, A. and Herranz, J.M., 2011. Does aboveground vegetation composition resemble soil seed bank during succession in specialized vegetation on gypsum soil?. Ecological Research, 27(1): 43-51.

- Mesdaghi, M., 2005. Plant Ecology. Jahade Daneshgahi of Mashhad Press, Mashhad, 187p (In Persian).

- Mesgaran, M.B., Mashhadi, H.R. and Alizadeh, H.M., 2007. Comparison of three methodologies for efficient seed extraction in studies of soil weed seedbanks. Weed Research, 47(6): 472-478.

- Morellato, L.P.C. and Haddad, C.F.B., 2000. Introduction: the Atlantic forest. Biotropica, 32(4b): 786-792.

- Noraiy, A., Jalali, S.Gh. and Esmailzadeh, O., 2014. Seasonal variation patterns of the soil seed bank in Populus Caspica protected area. Journal of Wood & Forest Science and Technology, 21(1): 1-20 (In Persian).

- Poiani, K.A. and Johnson, W.C., 1988. Evaluation of the emergence method in estimating seed bank composition of prairie wetlands. Aquatic Botany, 32(1-2): 91-97.

- Price, J.N., Wright, B.R., Gross, C.L. and Whalley, W.R.D.B., 2010. Comparison of seedling emergence and seed extraction techniques for estimating the composition of soil seed banks. British Ecological Society, Methods in Ecology and Evolution, 1(2): 151-157.

- Rahman, A., James, T.K. and Grbavac, N., 2001. Potential of weed seedbanks for managing weeds: a review of recent New Zealand research. Weed Biology and Management,1(2): 89-95.

- Rechinger, K.H., 1963-1998. Flora Iranica. Akademische Druck and Verlagsanstalt, Austria, Graz, Volume 1-173.

- Smutny', V. and Kr'en, J., 2002. Improvement of an elutriation method for estimation of weed seedbank in the soil. Rostlinna' Vy'roba, 48(6): 271-278.

- Ter Heerdt, G.N.J., Verwey, G.L., Bekker, R.M. and Bakker, J.P., 1996. An improved method for seed bank analysis: seedling-emergence after removing the soil by sieving. Functional Ecology, 10(1): 144-151.

- Thompson, K., Bakker, J. and Bekker, R., 1997. The Soil Seed Banks of North West Europe: Methodology, Density and Longevity. Cambridge University Press, Cambridge, 288p.

- Traba, J., Levassor, C. and Peco, B., 1998. Concentrating samples can lead to seed losses in soil bank estimations. Functional Ecology, 12: 975-976.

- Tsuyuzaki, SH. and Goto, M., 2001. Persistence of seed bank under thick volcanic deposits twenty years after eruptions of Mount Usu, Hokkaido Island, Japan. American Journal of Botany, 88(10): 1813-1817.