اثر سرمای دیررس شبانه بر اجزای فتوسیستم دو در سه کلن شالک (Populus nigra L.)

نوع مقاله: علمی- پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد جنگل‌داری، دانشکده کشاورزی، دانشگاه یاسوج

2 استادیار، گروه جنگل‌داری، دانشکده کشاورزی و پژوهشکده منابع طبیعی و محیط زیست، دانشگاه یاسوج

چکیده

ارزیابی عملکرد فتوسیستم دو به‌عنوان پرانرژی‌ترین فتوسیستم که قادر به شکستن آب و تأمین الکترون مورد نیاز مرحله نوری فتوسنتز است، از اهمیت ویژه‌ای برخوردار است. سرمای دیررس که به‌طور معمول در فصل رویش و در طول شب اتفاق می‌افتد، با آسیب رساندن به اندام‌های در حال رشد گیاه، موجب ضعف و حتی مرگ آنها می‌شود. در این پژوهش به‌منظور بررسی اثر سرمای دیررس شبانه بر اجزای عملکردی فتوسیستم دو و برخی از ویژگی‌های فیزیولوژیکی مرتبط با آن، نهال‌های سه کلن شالک (Populus nigra L.) به‌مدت سه شب در معرض حداقل دماهای شبانه 16، چهار، صفر و 20- درجه سانتی‌گراد قرار گرفتند. سپس نهال‌ها به‌مدت 14 روز در دمای بهینه به‌منظور بررسی توان احیا نگهداری شدند. داده‌ها با استفاده از مدل فاکتوریل با دو عامل کلن در سه سطح و حداقل دمای شبانه در چهار سطح به‌تفکیک برای مرحله تنش و احیا تجزیه و تحلیل شدند. نتایج نشان داد که عملکرد ماکزیمم و مؤثر فتوسیستم دو تحت تأثیر کارایی کمپلکس تجزیه‌کننده آب و فعالیت مخزن پلاستوکوئینون در کلن‌های مختلف روند کاهشی متفاوتی را نشان داد، به‌نحوی‌که کلن ایرانی حساس‌تر از کلن‌های دیگر بود. این تفاوت در ارتباط با محتوای قند محلول، پرولین، کلروفیل و نرخ نشت الکترولیت‌ها از غشای سیتوپلاسمی بررسی شد. همچنین روند بهبودی کلن‌ها در دوره احیا مقایسه شد.

کلیدواژه‌ها


عنوان مقاله [English]

Impacts of night late frost on photosystem II components of three black poplar (Populus nigra L.) clones

نویسندگان [English]

  • Ma'soumeh Hasanvand 1
  • Payam Fayyaz 2
1 M.Sc. Student Forestry, Faculty of Agriculture, Yasouj University
2 Assistant Prof., Department of Forestry, Faculty of Agriculture and Institute of Natural Resources and Environment, Yasouj University
چکیده [English]

It is of crucial importance to assess the performance of the photosystem II efficiency, as the most powerful system in light reactions of photosynthesis that is able to provide required electron of system by splitting water. Late frost occurred mostly in growing season and during night in early spring often results in weakness and even death of plants. Therefore, selecting tolerant varieties is one of the most efficient methods to deal with late frost. Due to high diversity and wide dispersal, poplars significantly contribute to the worldwide supply of cellulosic resources. In order to investigate the effect of night late frost on functional component of photosystem II and a number of related physiological traits, seedlings of three clones of black poplar (Populus nigra L.) were exposed to the minimum night temperature of 16, 4, 0 and –20 °C for three nights. To assess the revival potential, seedlings were further maintained in optimum temperature for 14 days. Data were analyzed using a factorial model with two factors of clone and minimum night temperature for each step of the stress and revival. Results revealed that maximum and quantum yield efficiency of photosystem II in different clones has different reduction patterns according to the efficiency of water splitting complex and plastoquinon pool (PQ). Thus, Iranian clone was concluded to be more sensitive than other clones. This difference was discussed in association with total soluble sugar, proline and chlorophyll concentration and electrolyte leakage rate of cytoplasmic membrane. In addition, the recovery process of the clones in revival period was discussed.

کلیدواژه‌ها [English]

  • Chilling
  • chlorophyll fluorescence
  • Freezing
  • plastoquinon pool activity
  • water splitting complex

- Chen, Y.Y. and Chen, Y.Z., 2007. Cold Acclimation-Induced changes in freezing resistance, the contents of soluble protein and proline and antioxidant enzyme activities in Populus euphratica calli. Shandong Agricultural Sciences, 3: 46-49.

- Christersson, L., von Fircks, H. and Sennerby-Forsse, L. 1983. Frost Hardiness Development and Frost Injuries of the Genus Salix, A Literature Review. Published by Ontario Tree Improvement and Forest Biomass Institute, Ministry of Natural Resources, Maple, Ontario, Canada.

- Estrella, N. and Menzel, A., 2014. Experimental assessment on the frost sensitivity during leaf development of juvenile Fagus sylvatica L. Abstracts of European Geosciences Union General Assembly Conference. Austria, 27 April to 2 May. 2014: Vol. 16, pp: 13152.

- Galiba, G., Erepesi, I.K., Snape, J.W. and Sutka, J., 1997. Location of a gene regulating cold-induced carbohydrate production on chromosome 5 of wheat. Journal of Theoretical and Applied Genetics, 95: 265-270.

- Ghasemi, R., Asadi F. and Torabi, A., 2010. Evaluation of height and diameter growth of indigenous and exotic poplar clones in one growing season. Iranian Journal of Forest, 1(4): 333-343 (In Persian).

- Glenn, E.P., Brown, J.J. and Blumwald, E., 1999. Salt tolerance and crop potential of halophytes. Journal of Plant Science, 18: 227-255.

- Hällgren, J.E. and Öquist, G. 1990. Adaptations to low temperatures: 265-293. In: Alscher, R.G. and Cumming, J.R., (Eds.). Stress Responses in Plants: Adaptation and Acclimation Mechanisms. Wiley-Liss, New York.

- Hirsh, A., Bent, T. and Erbe, E., 1989. Localization and characterization of intracellular liquid-liquid phase separations in deeply frozen Populus using electron microscopy, dynamic mechanical analysis and differential scanning calorimetry. Thermochimica Acta, 155: 163-186.

- Irigoyen, J.J., Emerich, D.W. and Sanchez-Diaz, M., 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa L.) plants. Physiologia Plantarum, 84: 55-60.

- Kazemi Shahandashti, S.S., Maali Amiri, R., Zeinali, H., Ramezanpour, S.S., Mahdieh, M. and Tabatabaifar, S.A., 2013. Assessment of gene expression pattern of rubisco and some physiological characteristics under cold stress condition in chickpea. Journal of Agricultural Biotechnology, 5(1): 145-157 (In Persian).

- Kerepesi, I., Bányai-Stefanovits, E. and Galiba, G., 2004. Cold acclimation and abscisic acid induced alterations in carbohydrate content in calli of wheat genotypes differing in frost tolerance. Journal of Plant Physiology, 161: 131-133.

- Kudoh, H. and Sonoike, K., 2002. Irreversible damage to photosystem I by chilling in the light: cause of the degradation of chlorophyll after returning to normal growth temperature. Journal of Planta, 215: 541-548.

- Maestrini, P., Cavallini, A., Rizzo, M., Giordani, T., Bernardi, R., Durante, M. and Natali, L., 2009. Isolation and expression analysis of low temperature-induced genes in white poplar (Populus alba). Journal of Plant Physiology, 166(14): 1544-1556.

- Man, R., Kayahara, G.J., Dangand, Q.L. and Rice, J.A., 2009. A case of severe frost damage prior to bud break in young conifers in Northeastern Ontario: Consequence of climate change? Forestry Chronicle, 85(3): 453-462.

- Matysik, J., Alia- Bhalu, B. and Mohanty, P., 2002. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Journal of Current Science, 82: 525-532.

- Oliveira, G. and Penuelas, J., 2000. Comparative photochemical and phenomorphological responses to winter stress of an evergreen (Quercus ilex L.) and a semi-deciduous (Cistus albidus L.) Mediterranean woody species. Journal of Acta Oecologica, 21(2): 97-107.

- Örlander, G., 1993. Shading reduces both visible and invisible frost damage to Norway spruce seedlings in the field. Forestry, 66(1): 27-36.

- Paeizi, M. and Shariati, M., 2012. Effect of cold stress on PSII efficiency of Dunaliella using chlorophyll a fluorescence kinetics. Journal of Cell & Tissue, 2(4): 395-405 (In Persian).

- Paquine, R. and Lechasseur, P., 1979. Observation sur une methode dosage la libre dans les de plantes. Journal of Botany, 57: 1851-1854.

- Sabeti, H., 1994. Forests, Trees and Shrubs of Iran. Second edition, Yazd University Press, Yazd, 810p (In Persian).

- Saeedi, Z. and Azadfar, D., 2011. Comparison among different clones of Populus nigra in aspect of cold resistance. Wood and Forest Science and Technology, 17(3): 99-111 (In Persian).

- Sahragard, N., 2007. Chilling (Freezing) and Ice-nucleating Bacteria in Plants. Published by Agricultural Research, Education and Extension Organization, Tehran, 116p (In Persian).

- Singh, D.P., 2003. Stress Physiology. New Age International Pvt Ltd Publishers, New Delhi, 184p.

- Taiz, L. and Zeiger, E., 2002. Plant Physiology, Published by Sinauer Associates, Sunderland, UK, 690p.

- Tsarouhas, V., Kenney, W.A. and Zsuffa, L., 2001. Variation in freezing resistance during different phenological stages in some Populus and Salix clones: Implications for clonal selection. Silvae Genetica, 50(2): 54-63.

- van Heerden, P.D.R., Krüger, G.H.J., Loveland, J.E., Parry, M.A.J. and Foyer, C.H., 2003. Dark chilling imposes metabolic restrictions on photosynthesis in soybean. Journal of Plant, Cell and Environment, 26(2): 323-337.‏

- Verwijst, T., Elowson, S.S., Li, X. and Leng, G., 1996. Production losses due to a summer frost in a Salix viminalis short-rotation forest in southern Sweden. Scandinavian Journal of Forest Research, 11: 104-110.

- Wang. Z., Quebedeuux, B. and Stutte, G.W., 1996. Partitioning of (14c) glucose into sorbitol and other carbohydrates in apple under water stress. Australian Journal of Plant Physiology, 23: 245-251.      

- Yelenosky, G., 1979. Accumulation of free proline in citrus leaves during cold hardening of young trees in controlled temperature regimes. Journal of Plant Physiology, 64: 425-427.

- Zobayed, S., Afreen, F. and Kozai, T., 2005. Temperature stress can alter the photosynthetic efficiency and secondary metabolite concentrations in St. John’s Wort. Plant Physiology and Biochemistry, 43: 977-984.