In vitro regeneration of desert teak (Tecomella undulata (Sm.) Seem); Investigating somatic embryogenesis potential of ovary explant

Document Type : Research article

Authors

1 Corresponding author, Assistant Prof., Department of Horticultural Science and Engineering, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, Iran

2 Assistant Prof., Department of Genetics and Plant Breeding, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, Iran

Abstract

     Tecomella undulata (Sm.) Seem known as desert teak, a multipurpose woody species has fallen under the category of endangered plants mainly due to lack of an efficient natural reproduction system. Inherent low adventitious rooting of cuttings has been the principal cause of failure in its vegetative propagation. Hence, research was conducted to assess in vitro somatic embryogenesis potential in the species. Ovary explant was cultured in modified MS media fortified with various auxins and cytokinins. The results revealed that NAA was superior in the induction of embryogenic callus (EC). NAA at 0.5, 2, 3 and 4 mg L-1 induced the highest ECs exhibiting developing pro-embryogenic masses and globular somatic embryos. TDZ and BA, though induced good callogenesis at low concentrations but the formed calli were non-embryogenic. The proliferation of embryogenic calli was the best on a phytohormone-free medium. The media containing NAA at 7.5 and 10 mg L-1 NAA only, induced somatic embryos alongside callus proliferation. The media containing low concentrations of BA (0.2 and 1 mg L-1) resulted in recurrent embryogenesis. Application of BA and GA3, and elevating the sucrose concentration of the culture medium did not make advancement in the maturation of the somatic embryos formed during stages of callogenesis and callus proliferation. The findings achieved in this study in the field of induction of EC and somatic embryo can be a beneficial basis for future extensive studies on micropropagation of this recalcitrant species.

Keywords

References

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