Effect of different factors on micropropagation of Abarkooh 4000-year-old horizontal cypress (Cupressus sempervirens L. var. horizontalis (Mill.) Gord.)

Document Type : Research article

Authors

1 M.Sc. Student, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, Iran

2 Assistant Prof., Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, Iran

3 Assistant Prof., Department of Soil Science, Faculty of Natural Resources, Yazd University, Yazd, Iran

Abstract

This study was conducted to protect the genetic properties and propagation of 4000-year-old Abarkooh horizontal cypress (Cupressus semprvirens L. var. horizontalis (Mill.) Gord.) by tissue culture technique. In the first experiment, the effect of preparation time of explant (four seasons), medium culture (MS and WPM) and concentrations of BA (0, 0.1 and 1 mg/l) on proliferation of horizontal cypress explants was investigated. In second and third experiments the effect of culture media (MS and WPM), concentrations of BA (0, 0.1, 0.5 and 1 mg/l) was studied alone or in combination with 0.01 mg/l IBA and type of explant (primary or secondary shoots) in a factorial experiment and completely randomized design with three replications. In first experiment, the maximum shoots number was obtained from spring and summer explants that were cultured in MS and WPM culture media, respectively. In second experiment, the maximum number and length of shoots was obtained in WPM culture medium and secondary shoots. The highest length of shoots was found in first shoots cultured in WPM medium containing 0.1 and 0.5 mg/l BA, followed by primary shoots cultured in MS medium culture without BA. In the third experiment, the maximum number of shoots (3) was observed in secondary shoots by using WPM medium containing 0 mg/l BA + 0.01 mg/l IBA. Conclusively the best time of preparing explant, type of culture media and explant were found to be spring, WPM medium and secondary shoots, respectively.

Keywords


- Aghbash, F.Gh., Jalali, Gh.A.,  Teymourzadeh, A. and Hosseini, M., 2006. A quantitative and qualitative study of Picea excelsa and Pinus nigra in Fandoghloo forest reserve. Journal of Forest and Wood Products (Iranian Journal of Natural Resources), 59(1): 131-138 (In Persian).
- Zaidi, M.A., Khan, S., Jahan, N., Yousafzai, A. and Mansoor, A., 2012. Micropropagation and conservation of three Juniperus specices (Cuperssaceae). Pakistan Journal of Botany, 44: 301-304.
- Bhojwani, S.S. and Razdan, M.K., 1996. Plant Tissue Culture: Theory and Practice. Elsevier, Amsterdam, 767p.
- Bolandi, A.R., Hamidi, H. and Rezagholy, A.A., 2016. Effects of culture media and growth regulators on propagation of rootstock GF677 in tissue culture conditions. Journal of Plant Researches, 29(1): 1-14 (In Persian).
- Burrows, G.E., Doley, D.D., Haines, R.J. and Nikles, D.G., 1988. In vitro propagation of Araucariacunninghamii and other species of the Araucariaceae via axillary meristems. Australian Journal of Botany, 36(6): 665-676.
- Capuana, M. and Giannini, R., 1997. Micropropagation of young and adult plants of cypress (Cupressus sempervirens L.). Journal of Horticultural Science, 72(3): 453-460.
- Fossi, D., Di Paola Lipucci, M. and Tognoni, F., 1981. Induzione invitrodi gemme ascellari della specie "Cupressus sempervirens" (L.). Rivista di Ortoflorofrutticoltura Italiana, 65(4): 293-299.
- Giovanelli, A., De Carlo, A., 2007. Micropropagation of Mediterranean cypress (Cupressus sempervirens L.): 93-105. In: Mohan Jain, S. and Häggman, H., (Eds.). Protocols for Micropropagation of Woody Trees and Fruits. Springer, Netherlands, 559p.
- Hussain, A., Qarshi, I.A., Nazir, H., Uliah, I., Rashid, M. and Shinwari, Z.Kh., 2013. In vitro callogenesis and organogenesis in Taxus wallichiana Zucc. the Himalayan yew. Pakistan Journal of Botany, 45(5): 1755-1759.
- Irannejad Parizi, M.H., Akbari, H., Khoshnevis, M., Shams, M.R., Abedini, T., Rad, M.H., Rasouli, S.A.R., Taheri, A. and Mahdavi, M.R., 2016. National Monument of Abarkooh Cypress. Yazd University Press, Yazd, 308p (In Persian).
- Karimpour, S., Davarynejad, G.H., Bagheri, A. and Tehranifar, A., 2013. In vitro establishment and clonal propagation of Sebri pear cultivar. Journal of Agricultural Science and Technology, 15: 1209-1217.
- Khawar, K.M., Sarihan, E.O., Sevimay, C.S., Çöçü, S., Parmaksiz, I., Uranbey, S., Ipek, A., Kaya, M.D., Sancak, C. and Özcan, S., 2005. Adventitious shoot regeneration and micropropagation of Plantago lanceolata L. Periodicum Biologorum, 107(1): 113-116.
- Khoshnevis, M., Matinizadeh, M., Shirvany, A. and Teimouri, M., 2017. Iran, the treasure of long-lived trees. Iran Nature, 2(4): 42-55 (In Persian).
- Mahdinejad, N., Fakheri, B.A. and Ghanbari, S., 2015. Effect of growth regulators on in vitro callogenesis Taxus baccata L. Biological Forum - An International Journal, 7(1): 142-145.
- McCown, B.H. and Lloyd, G., 1981. Woody plant medium (WPM) - A mineral nutrient formulation for microculture for woody plant species. HortScience, 16: 453-453.
- Misson, J.P., 1988. Multiplication du Thuja plicata par culture in vitro de tissus juvéniles et  âgés. Canadian Journal of Forest Research, 18(4): 473-477.
- Morte, M.A., Honrubia, M. and Piqueras, A., 1992. Micropropagation of Tetraclinis articulata (Vahl) Masters (Cupressaceae). Plant Cell, Tissue and Organ Culture, 28: 231-233.
- Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
- Neibaur, I., Gallo, M. and Altpeter, F., 2008. The effect of auxin type and cytokinin concentration on callus induction and plant regeneration frequency from immature inflorescence segments of seashore paspalum (Paspalum vaginatum Swartz). In Vitro Cellular & Developmental Biology - Plant, 44: 480-486.
- Rahmati, Z., Payam Nour, V., Ghasemi Bezdi, K. and Ebrahimi, P., 2017. Optimization of culture medium for in vitro callogenesis in Taxus baccata L. and T. berevifolia Nutt. Journal of Forest and Wood Products (Iranian Journal of Natural Resources), 70(3): 381-391 (In Persian).
- Razavi, R.S., 2013. Proliferation induction of shoot and root in in vitro for micropropagation of Juniperus polycarpos C. Koch. M.Sc. thesis, Faculty of Agricultural Science, Payame Noor University, Tehran, 113p (In Persian).
- Sepahvand, S., Ebadi, A., Kamali, K. and Ghaemmaghami, S.A., 2012. Effect of myo-inositol and thiamine on micropropagation of Gf677 (Peach × Almond Hybrid). Journal of Agricultural Science, 4(2): 275-280.
- Taghipoor, M., Haddad, R. and Ghannadnia, M., 2015. The effect of media, explants and cytokinin on micropropagation of Araucaria excelsa R. Journal of Applied Crop Breeding, 3(1): 1-12 (In Persian).
- Thomas, M.J., Duhoux, E. and Vazart, J., 1977. In vitro organ initiation in tissue culture of Biota orientalis and other species of the Cupressaceae. Plant Science Letters, 8(4): 395-400.
- Yari, M., Gholami, M. and AsnaAshari, M., 2011. Effect of sampling time, explant type, culture orientation and antioxidant type on in-vitro explant establishment and growth of Persian Walnut. Iranian Journal of Horticultural Science, 42(2): 141-149 (In Persian).
- Zabihi, H., Hosseni Nasr, S.M., Babaeyan Jelodar, N. and Jalilvand, H., 2008. Tissue culture of Ulmus glabra Hudson: The effects of culture media, explants and their harvest times. Journal of Agricultural Science and Natural Resources, 15(4): 15-21 (In Persian).