Effect of cutting time, cutting length and hormone on rooting and germination of Vitex pseudo-negundo (Hausskn.) Hand-Mzt. cuttings

Document Type : Research article

Authors

1 M.Sc. student Forest Ecology and Silviculture, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran

2 Associate Prof., Department of Forestry, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran

3 Associate Prof., Department of Forestry and Forest Economics, Faculty of Natural Resources, University of Tehran, Karaj, Iran

4 M.Sc. Horticultural Science, Behbahan Municipality, Behbahan, Iran

Abstract

Vitex pseudo-negundo (Hausskn.) Hand-Mzt. is a shrub that is height of about 1-2 meters. To study vegetative propagation of Vitex pseudo-negundo experimental was conducted using a‌ 6 × 3 × 2 factorial design based on completely randomized design with 4 replications and 30 cuttings per treatment in Behbahan municipalities' greenhouse. The experiment factors included: Cutting time at 6 levels (22 December and 5 January, 21 January and 4 February, 20 February and 6 March), Cutting length at 3 levels (15, 20 and 25 cm) and NAA hormone at 2 levels (with hormones and hormone-free). Results showed that the cuttings taken in 21 January, 20 February and 6 March have the highest rooting and germination percent and root number. The highest (rooting and germination percent) and (root number and root dry and fresh weight) were observed in length cuttings of 15, 20 cm and 25 cm respectively. The interaction effect of hormone and cutting time and cutting length, indicator being more dry weight, fresh weight and root diameter in cuttings with hormone taken in 20 February with a length of 25 cm. The use of hormone has the negative effect on rooting and germination percent and root length. In this study, the best result was achieved for cuttings taken in 20 February, with a length of 25 cm and without the use of hormones.

Keywords


- Abdou, M.A., Mohamed, M.A.H. and Attia, F.A., 2004. Physiological studies on Ficus benjamina plants. 1: Effect of cutting collection, IBA and nofatrein on chemical composition, root ability of cutting and transplants growth. Mansoura University Journal of Agricultural Science, 29: 775-785.
- Aboutalebi, A. and Tafazoli, E., 2006. Effects of cutting time and auxin on rooting of sweet lime (Citrus limetta L.). Journal of Agricultural Sciences and Natural Resources, 13(5): 29-37 (In Persian).
- Asadi, F. and Ghasemi, R., 2007. Evaluation of rooting success in poplar clones cuttings using different treatments. Iranian Journal of Forest and Poplar Research, 15(2): 134-143 (In Persian).
- Bagheri, R., Ghasemi, R. and Merrikh, F., 2010.  Determination of appropriate place of cutting in shoots and young branches of five poplar species and clones. Iranian Journal of Forest and Poplar Research, 18(4): 621-629 (In Persian).
- Bartolini, G., Fabbri, A. and Tathini, M., 1986. Effects of phenolic acids and auxin on rooting of Olea europaea L. cuttings. Horticultural Science, 21(3): 662-672.
- Basiri, R., Taleshi, H., Poorrezaee, J., Hassani, S.M. and Gharehghani, R., 2011. Flora, life form and chorotypes of plants in river forest Behbahan, Iran. Middle-East Journal of Scientific Research, 9(2): 246-252.
- Bhattacharjee, S.K. and Balakrishna, M., 1986. Standardization of propagation of Hibiscus rosa-sinensis L. from stem cuttings. South Indian Horticulture, 34: 158-166.
- Dako, E.G.A., Danikou, S., Tchokponhoue, D.A., Komlan, F.A., Larwanou, M., Vodouhe, R.S. and Ahancheded, A., 2014. Sustainable use and conservation of Vitex doniana sweet: unlocking the propagation ability using stem cutting. Journal of Agriculture and Environment for International Development, 108(1): 43-62.

- Emami, A., 1996. Methods of Plant Analysis. Published by Soil & Water Research Institute, Karaj, 128p (In Persian).

- Ersoy, N. and Aydin, M., 2008. The effect of some hormone and humidity levels on rooting of Mahaleb (Prunus mahaleb) soft wood top cutting. Suleyman Demired Universitesi Ziraat Fakultesi Degisi, 3(1): 32-41.
- Haile, G., Gebrehiwot, K., Lemenih, M. and Bongers, F., 2011. Time of collection and cutting sizes affect vegetative propagation of Boswellia papyrifera (Del.) Hochst through leafless branch cuttings. Journal of Arid Environments, 75: 873-877.
- Hartmann, H.T., Kester, D.E., Davies, F.T. and Geneve, R.L., 1997. Plant Propagation, Principles and Practices. Prentice-Hall, Englewood Cliffs, New Jersey, 393p.
- Ibironke, O.A., 2013. The effects of cutting types and length on rooting of Duranta repens in the Nursery. Global Journal of Human-Social Science Research, 13(3): 1-5.
- Khoshkhoy, M., 2007. Plant Propagation (translation). Shiraz University Press, Shiraz, 550p (In Persian).
 - Kochhar, S., Singh, S.P. and Kochhar, V.K., 2008. Effect of auxins and associated biochemical changes during clonal propagation of the biofuel plant–jatropha curcas. Biomass and Bioenergy, 32: 1136-1143.
- Mozaffarian, V., 2005. Trees and Shrubs of Iran. Published by Farhang Mo`aser, Tehran, 1120p (In Persian).
- Ozel, C.A., Khawar, K.H.M., Mirici, S., Arslan, O. and Ozcan, S., 2006. Induction of ex vitro adventitious roots on soft wood cuttings of Centaurea tchihatcheffii Fisch et. Mey. using Indole 3-Butyric Acid and α-Naphthalene Acetic Acid. International Journal of Agriculture and Biology, 8(1): 66-69.
 - Palanisamy, K. and Kumar, P., 1997. Effect of position, size of cuttings and environmental factors on adventitious rooting in neem (Azadirachta indica A. Juss.). Forest Ecology andManagement, 98(3): 277-280.
- Reezi, S., Naderi, R., Khalighi, A., Zamani, Z. and Etemad, V., 2006. Asexual propagation of Picea pungens Koster through cuttings, and grafting under various hormonal treatments. Iranian Journal of Natural Resources, 59(3): 589-601 (In Persian).
- Tandon, V.R. and Guota, R.K., 2005. Anexperimental evaluation of anticonvulsant activity of Vitex-negundo. Indian Journal of Physiology and Pharmacology, 49(2): 199-205.
- Tchinda, N.D., Messi, H.J.C.M., Fotso, Nzweundji, G., Tsabang, N., Dongmo, B., Oumar, D., Tarkang, P.A., Caver A. and Ndoumou, D.O., 2013. Improving propagation methods of Ricinodendron heudelotti Baill. from cuttings. South African Journal of Botany, 88: 3-9.
- Teale, W.D., Paponov, I.A., Ditengou, F. and Palme, K., 2005. Auxin and the developing root of Arabidopsis thaliana. Physiologia Plantarum, 123: 130-138.
- Troncoso, A., Montano, J.C., Murillo, J.M. and Cantos, M., 1988. Effect of photoperiod on the growth and mineral composition of young olive plants. Olea, 19: 63-67.