Biology and physiology of sexual reproduction in Pistacia atlantica subsp. mutica

Document Type : Scientific article

Author

Scientific Board Member, Research Institute of Forests and Rangelands

Abstract

Pistacia atlantica subsp. mutica is a predominant and endemic species throughout the western sparse forests of Iran. In this study, some of the biological and physiological aspects such as flower and fruit development, embryo sac and endosperm initiation, embryo development and resting periods, up to 16 weeks after anthesis as well as pollen tube growth and development along a week of fertilization, were studied.
The collected samples were fixed in Carnoy's solution for pollen tube growth. Tissues for embryo sac, embryo and endosperm studies were fixed in FAA and stored in 75% ethanol in the fridge (at 4˚ C) up to the processing time. Pollen tube was stained using aniline blue dye and samples were studied with a light microscope facilitated with an epi-florescence adaptor with 3 barrier filters, under UV light reflection. All samples for assessing the tissues were dissected 3-7 micrometers and then stained with toluodine blue O (TBO), Schiff’s reagent and periodic acid. Preparations were observed under a light Zise microscope. Data were analyzed by SAS analytical program, using a nested design and excel software for comparison means analysis.

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References

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Iranian Journal of Forest and Poplar Research
Volume 12, Issue 3
October 2004
Pages 412-391

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