A preliminary study on resin duct initiation and development in one-year-old seedlings of Pistacia atlantica subsp. mutica

Document Type : Research article

Authors

Members of Scientific Board , Research Institute of Forests and Rangelands

Abstract

Natural gum and resins are basic materials required by different industries, including food, pharmaceutical, cosmetic, painting, etc.  They are extracted from various plants such as coniferous, citrus, elm, oleaster, apricot and wild pistachio species.  The main problem is that the gum should be extracted from mature and aged trees. Cutting the trees bark and some times the young wooden layer of the stem or young branches, is one of the methods of extracting gum and resin in these trees.  Insects bit and bark damage by some fungi also is a way to extract gums, because the trees produce gum or resin against disease contamination.  In that case, the plants are very aged and weak.  Thus, gum and resin exudation, particularly when is repetitive, can lead to general plant weakness and finally to its death..
It was tried to obtain extract from young seedlings of Pistacia atlantica in this research program which require a preliminary study of resin and gum ducts physical structure and their growth and development process.  Resin ducts were studied in root, shoot and leaf by picking one-year organ samplings.  Samples were fixed with FAA fluid and kept in fridge (4- 8 °C) for a while.  Then the samples were hydrated with an alcohol series and cut using Rm-2035 microtome.  five to 10 thick sections were stained with periodic acid and Schiff’s reagent (PAS) and toluidine blue O.  Preparations were studied under a light microscope and photographs were taken using 100 ISO Konica Minolta color film.
As a result, the number of gum duct in the bark of root, stem and leaf was discriminated in Pistacia atlantica seedlings.  The results showed that it is possible to identify different genotypes of Pistacia atlantica through this differentiation.  Moreover, it might be demonstrated that despite of former idea for resin production of seedlings, it is possible to have resin product at seedling stage.

Keywords

References

- Copeland, F., H., 1955. The reproductive structures of Pistacia chinensis (Anacardiaceae). Phytomorphology. 5: 441-449.
- Fahn, A., 1988. Tansley revew no. 14  Secretory tissue in vascular plants. New Phytologists.  108: 229-257.
- Franceschi, R., V., Krekling, T., Berryaman, A., A., and Christiansen, E., 1998.  Specialized phloem parenchyma cells in Norway spruce (Pinaceae) bark are an important site of defense reactions.  .  American Journal of Botany.  85(5): 601-615.
- Franceschi, V., R., Krokene, P., Krekling, T., and Christiansen, E., 2000.  Phloem parenchyma cells are involved in local and distant defense responses to fungal inoculation or bark-beetle attack in Norway spruce (Pinaceae).  American Journal of Botany, 87: 314-326.
- Lombardero, J., M., Yares, P., M., and Yares, B., 2005.  Effects of fire and mechanical wounding on Pinus resinosa resin defenses, beetle attacks and pathogens.  Forest ecology and management.  24: 1-16.
- Morrison, J., C., and Polito, V., S., 1985.  Gum duct development in almond fruit, Prunus dulcis (Mill.) D. A. Webb.  Botanical Gazette.  146(1): 15-25.
- Nagy, N., E., Franceschi, V., R., Solheim, H., Krekling, T., and Christiansen, E., 2000.  Wound-induced traumatic resin duct development in stems of Norway spruce (Pinaceae) : anatomy and cytochemical traits.  American Journal of Botany.  87: 302-313.
- Pain, T., D., Raffa, K., F., Harrington, T., C., 1997.  Interactions among scolytid bark beetles their associated fungi, and live hosts conifers.  Annals Review of Entomology.  47: 179-206.
- Percival, G., C., 2001.  Induction of systemic acquired disease resistance in plants: Potential implications for disease management in urban forestry.  Journal of Arboriculture.  27(4): 181-192.
- Rajput, K., S., Rao, S., K., and Vyas, H., P., 2005.  Formation of gum ducts in Azadirachta indica A. juss.  Journal of Sustainable Forestry.  20 (2): 1-13.
- Sheue, C., R., Yang, Y., P., and Huo-Huang, L., L., 2003.  Altitudinal variation of resin ducts in Pinus taiwanensis Hayata (Pinaceae) needles. Botanical Bulletin of Academic Sinica.  44: 305-313.
- Shuraki, Y., D., and Sedgley, M., 1997.  Pollen tube pathway and stimulation of embryo sac development in Pistacia vera (Anacardiaceae).  Annals of Botany.  79: 361-369.
 
 

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