Genetical variation on natural populations of Populus euphratica Oliv. by peroxidase isoenzyme

Document Type : Research article

Authors

1 Member of scientific board, Research Institute of Forests and Rangelands

2 Member of scientific board, Tarbiat Modarres University.

Abstract

Populus euphratica Oliv. distributed naturally in vast regions of Iran. Also it is a native species in arid and semi arid of the country. Geographical and climatical differences in P. euphratica sites affect on the morphological and genetical differences among the tree populations. Peroxidase isoenzyme analysis from leaf extracts of P. euphratica using polyacrylamid gel electrophoresis was conducted to determine genetical characteristics and differentiate individuals in eleven natural populations. Results of peroxidase activity patterns in 110 trees from 11 provenances showed differences among individuals. Peroxidase banding pattern showed three zones of activity. Cluster analysis of data based on bands frequency for 11 provenances showed the existence of three distinct major groups, group 1: Sarakhs, Khojir and Dezful; group 2: Dashly-borun, Malavi, Hamidiyeh, Gotvand, Manjil and Mahneshan; group 3: Gherekhlar. Also there was more genetic distance between Sarkhs and Gherekhlar sites in eastern and northern zones respectively.

Keywords


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