Comparison of Pinus eldarica Medw. with its two natural generated forms using some biochemical and morphological traits

Document Type : Research article

Authors

1 M.Sc. in forestry

2 Associate Prof., Department of Forestry, Tarbiat Modares University

3 Assistant Prof., Department of Plant Science, Tarbiat Modares University

Abstract

 In order to distinguish the Mondell pine species (Pinus eldarica) from its two natural generated morphotypes (i.e. Ball-shaped and Conical-shaped pines), qualitative alteration of peroxidase enzyme and the assessment of the enzyme activity were compared by use of polyacrylamide electrophoretic method on PAGE system. Furthermore, lignin contents of the needle and stem samples along with 13 macro morphological characteristics of individuals were compared with each other. Results showed that the isozyme pattern of three types of the studied pines, the activity of this enzyme and lignin contents were similar. Whereas, the morphological traits comparison, indicate clear differences in the pines. These, state that despite of obvious morphological differences and application of peroxidase enzyme in physiological changes, plant evolution studies, genetic consideration and identifying genus, species and subspecies but at least soluble portion is not suitable marker for distinction of these pines. The results of this study also, propose to use of molecular markers and cytogenetically studies in order to specify the cause of this occurrence.

Keywords


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