رابطه قارچ‌‌های اکتومیکوریز با برخی ویژگی‌های شیمی خاک در راشستان‌‌های فریم، استان مازندران

نوع مقاله: علمی- پژوهشی

نویسندگان

1 دکترای جنگل‌شناسی و اکولوژی جنگل، گروه جنگل‌داری، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

2 دانشیار، گروه جنگل‌داری، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

3 دانشیار، گروه گیاه‌پزشکی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

4 استادیار، ایستگاه تحقیقات جنگل و مرتع پاسند، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان مازندران، سازمان تحقیقات، آموزش و ترویج کشاورزی، بهشهر، ایران

چکیده

با توجه به نقش مثبت قارچ‌‌های اکتومیکوریز در جذب آب و مواد غذایی و انتقال به درختان، شناسایی آن‌ها از اهمیت زیادی برخوردار است. این پژوهش با هدف بررسی ارتباط تنوع قارچ‌‌های اکتومیکوریز شناسایی‌شده با برخی ویژگی‌های شیمی خاک در سری فلورد جنگل‌های فریم استان مازندران انجام شد. در سه دامنه ارتفاعی 300 متری (1200 تا 1500، 1500 تا 1800 و 1800 تا 2100)، 45 درخت راش (Fagus orientalis Lipsky) (در هر طبقه ارتفاعی 15 درخت) انتخاب و نمونه‌‌برداری از ریزریشه‌‌های آن‌ها به‌عمق 10 سانتی‌‌متر انجام شد. در کنار محل مورد نظر برای برداشت ریشه، یک نمونه به ‌عمق 10 سانتی‌متر برای بررسی ویژگی‌های شیمیایی خاک نیز برداشت شد. نمونه‌‌های ریشه در آزمایشگاه قارچ‌‌شناسی از طریق استخراج DNA ناحیه ITS nrDNA با استفاده از زوج آغازگر‌های ITS1F و ITS4B یا ITS4 تکثیر و توالی‌یابی شده و با نرم‌افزار BioEdit اصلاح و در NCBI بررسی شدند. نمونه‌‌های خاک نیز پس از انتقال به آزمایشگاه، به‌مدت دو هفته در هوای آزاد خشک شده و پس از عبور از الک‌‌های دو میلی‌‌متری، مشخصه‌‌های شیمی خاک بررسی شدند. بر اساس نتایج بررسی همزیستی اکتومیکوریزی، 10 خانواده مختلف شناسایی شدند و بیشترین همزیستی مربوط به Russulaceae و Cortinariaceae بود. مقدار مشخصه‌‌های خاک مانند pH، نیتروژن، فسفر، پتاسیم و کربن در طبقه‌ ارتفاعی اول بیشتر از دو طبقه دیگر بود. همچنین، نتایج تجزیه واریانس و مقایسه‌های میانگین متغیرهای خاکی در منطقه مورد مطالعه نشان داد که بین طبقه‌های ارتفاعی از نظر مقدار فسفر، پتاسیم و اسیدیته تفاوت معنی‌‌داری وجود داشت. یافته‌های این پژوهش مؤید آن است که شرایط رویشگاهی و تنوع گونه‌‌ای توده‌های جنگلی می‌‌توانند بر ویژگی‌های خاک و غنای گونه‌‌ای اکتومیکوریزها و پیرو آن، پویایی بوم‌سازگان جنگل مؤثر باشند.

کلیدواژه‌ها


عنوان مقاله [English]

The relationship between ectomycorrhizal fungi and some soil chemical properties in beech stands of Farim, Mazandaran province

نویسندگان [English]

  • Hamed Aghajani 1
  • Seyyed Mohammad Hodjati 2
  • Mohammad Ali Tajick-Ghanbari 3
  • Mohammad Reza Pourmajidian 2
  • Ali Borhani 4
1 Ph.D. of Silviculture and Forest Ecology, Department of Forestry, Sari Agriculture Sciences and Natural Resources University, Sari, Iran
2 Associate Prof., Department of Forestry, Sari Agriculture Sciences and Natural Resources University, Sari, Iran
3 Associate Prof., Department of Plant Protection, Sari Agriculture Sciences and Natural Resources University, Sari, Iran
4 Assistant Prof., Pasand Forest and Rangeland Research Station, Agricultural and Natural Resources Research and Education Center of Mazandaran, AREEO, Behshahr, Iran
چکیده [English]

     According to the positive role and symbiosis of ectomycorrhizal fungi for absorbing water and nutrition and transferring to trees, identification of them are of great importance. The aim of this study was to investigate the relationship between recognized ectomycorrhizal fungi and some soil chemical properties in the Flourd series of Farim forests, Mazandaran province. Forty five beech trees were measured in three elevation classes (1200-1500, 1500-1800, and 1800-2100m a.s.l.) and samples were taken from the root tips at a depth of 10 cm. ITS nrDNA was replicated and sequenced using ITS1F and ITS4B or ITS4 primer pairs, then was corrected by BioEdit software and studied in NCBI. After specified the desired tree for sampling root tips, a sample was taken next to the same tree at a depth of 10 cm to examine of soil chemical properties. The results of this study showed that 10 different families were identified that the most symbiosis was in the Russulaceae and Cortinariaceae families. The results showed that edaphic factors such as soil pH, N, P, K, and C in the first elevation were higher than two other elevations. Moreover, the results of statistical analysis showed that P, K, and pH had a significant difference. Our results suggested that forest site conditions and tree diversity can influence on soil properties and the richness of ectomycorrhizal fungi and consequently on forest ecosystem dynamic.

کلیدواژه‌ها [English]

  • Forest health
  • Hyrcanian forests؛ phosphor؛ plant ecology؛ potassium؛ soil pH

- Agerer, R., 1987-2012. Colour atlas of ectomycorrhizae. Einhorn-Verlag, Schwäbisch Gmünd, Germany.

- Aghajani, H., Hojjati, S.M., Tajick-Ghanbari, M.A., Pourmajidian, M.R. and Borhani, A., 2017. Molecular identification of ectomycorrhizal fungal communities associated with oriental beech trees (Fagus orientalis Lipsky) in Hyrcanian forest of Iran. Iranian Journal of Science and Technology, Transactions A: Science, 42: 1-8.

- Ahmadi Delesam, T., Zarin Kafsh, M. and Sardabi, H., 2000. An investigation on the relationship between soil physical and chemical properties and its mineral nutrition in yew (Taxus baccata), Waz forest research (west Mazandaran state). Pajouhesh & Sazandegi, 13(2): 69-75 (In Persian).

- Alikhani, H.A. and Ghorchiani, M., 2012. Mycorrhizae: Sustainable Agriculture and Forestry (translation). Jahad-e Daneshgahi, Tehran, 373p (In Persian).

- Anonymous, 2010. Forest Management Plan of District Felord. Administration of Natural Resources and Watershed Management at Mazandaran province, Sari, 210p (In Persian).

- Bakker, M.R., Garbaye, J. and Nys, C., 2000. Effect of liming on the ectomycorrhizal status of oak. Forest Ecology and Management, 126(2): 121-131.

- Bruns, T.D., 1995. Thoughts on the processes that maintain local species diversity of ectomycorrhizal fungi. Plant and Soil, 170(1): 63-73.

- Craig, A.J., Woods, S. and Hoeksema, J.D., 2016. Influences of host plant identity and disturbance on spatial structure and community composition of ectomycorrhizal fungi in a northern Mississippi uplands ecosystem. FungalEcology, 24: 7-14.

- Engler, R.M. and Patrick, W.H. Jr., 1975. Stability of sulfides of manganese, iron, zinc, copper, and mercury in flooded and nonflooded soil. Soil Science, 119(3): 217-221.

- Fisher, M.A. and Fulé, P.Z., 2004. Changes in forest vegetation and arbuscular mycorrhizae along a steep elevation gradient in Arizona. Forest Ecology and Management, 200(1-3): 293-311.

- Gardes, M. and Bruns, T.D., 1993. ITS primers with enhanced specificity for basidiomycetes‐application to the identification of mycorrhizae and rusts. Molecular Ecology, 2(2): 113-118.

- Hagen-Thorn, A., Callesen, I., Armolaitis, K. and Nihlgård, B., 2004. The impact of six European tree species on the chemistry of mineral topsoil in forest plantations on former agricultural land. Forest Ecology and Management, 195(3): 373-384.

- Horton, B.M., Glen, M., Davidson, N.J., Ratkowsky, D., Close, D.C., Wardlaw, T.J. and Mohammed, C., 2013. Temperate eucalypt forest decline is linked to altered ectomycorrhizal communities mediated by soil chemistry. Forest Ecology and Management, 302: 329-337.

- Huang, J., Nara, K., Zong, K., Wang, J., Xue, S., Peng, K., Sheng, Z. and Lian, C., 2014. Ectomycorrhizal fungal communities associated with Masson pine (Pinus massoniana) and white oak (Quercus fabri) in a manganese mining region in Hunan province, China. Fungal Ecology, 9: 1-10.

- Ishida, T.A., Nara, K. and Hogetsu, T., 2007. Host effects on ectomycorrhizal fungal communities: insight from eight host species in mixed conifer-broadleaf forests. NewPhytologist, 174(2): 430-440.

- Jafari Haghighi, M., 2003. Methods of Soil Analysis: Sampling and Important Physical & Chemical Analysis with Emphasis on Theoretical & Applied Principles. Nedaye Zoha, Sari, 240p (In Persian).

- Jarvis, S.G., Woodward, S. and Taylor, A.F.S., 2015. Strong altitudinal partitioning in the distributions of ectomycorrhizal fungi along a short (300 m) elevation gradient. New Phytologist, 206(3): 1145-1155.

- Kazemi, Sh., 2014. The role of forest management on structure characteristics, understory plant diversity and soil properties in beech-hornbeam type (Case study: Khalilshahr forestry plan, district no. 1, parcels no. 10 and 12, Abbas Abad, Mazandaran province). M.Sc. thesis, Faculty of Natural Resources, Sari Agricultural Science and Natural Resources University, Sari, 81p (In Persian).

- Kianmehr, A., 2013. Investigation on stand growth characteristics, biodiversity and soil properties in natural pure and mixed beech and hornbeam forests (Case study: first district, Neka-Zalemrod forestry plan). M.Sc. thesis, Faculty of Natural Resources, Sari Agricultural Science and Natural Resources University, Sari, 113p (In Persian).

- Martin, F., Lapeyrie, F. and Tagu, D., 1997. Altered gene expression during ectomycorrhizal development: 223-242. In: Carroll, G.C. and Tudzynski, P. (Eds.). Plant Relationships, Part A. Springer-Verlag, Berlin, Heidelberg, 253p.

- Marvie-Mohadjer, M.R., 2011. Silviculture. University of Tehran Press, Tehran, 418p (In Persian).

- Miyamoto, Y., Sakai, A., Hattori, M. and Nara, K., 2015. Strong effect of climate on ectomycorrhizal fungal composition: evidence from range overlap between two mountains. The ISME Journal, 9: 1870-1879.

- Mostajeran, A. and Zoei, F., 2006. Mutualism: Mycorrhiza. University of Esfahan, Esfahan, 226p (In Persian).

- Newbound, M., Mccarthy, M.A. and Lebel, T., 2010. Fungi and the urban environment: A review. Landscape and Urban Planning, 96(3): 138-145.

- Rincón, A., Santamaría-Pérez, B., Rabasa, S.G., Coince, A., Marçais, B. and Buée, M., 2015. Compartmentalized and contrasted response of ectomycorrhizal and soil fungal communities of Scots pine forests along elevation gradients in France and Spain. Environmental Microbiology, 17(8): 3009-3024.

- Siddiqui, Z.A. and Pichtel, J., 2008. Mycorrhizae: an overview: 1-35. In: Siddiqui, Z.A., Akhtar, M.S. and Futai,K. (Eds.).Mycorrhizae: Sustainable Agriculture and Forestry. Springer, Netherlands, 359p.

- Smith, S.E. and Read, D.J., 2008. Mycorrhizal Symbiosis, Third Edition. Academic Press, New York, 803p.

- Solomon, D., Fritzsche, F., Lehmann, J., Tekalign, M. and Zech, W., 2002. Soil organic matter dynamics in the subhumid agroecosystems of the Ethiopian highlands: Evidence from natural 13C abundance and particle-size fractionation. Soil Science Society of America Journal, 66(3): 969-978.

- Steiner, M., Linkov, I. and Yoshida, S., 2002. The role of fungi in the transfer and cycling of radionuclides in forest ecosystems. Journal of Environmental Radioactivity, 58(2-3): 217-241.

- Suz, L.M., Barsoum, N., Benham, S., Dietrich, H.P., Fetzer, K.D., Fischer, R., García, P., Gehrman, J., Kristöfel, F., Manninger, M., Neagu S., Nicolas. M., Oldenburger, J., Raspe, S., Sánchez, G., Schröck, H.W., Schubert, A., Verheyen, K., Verstraeten, A. and Bidartondo, M.T., 2014. Environmental drivers of ectomycorrhizal communities in Europe's temperate oak forests. Molecular Ecology, 23(22): 5628-5644.

- Wang, X., Liu, J., Long, D., Han, Q. and Huang, J., 2017. The ectomycorrhizal fungal communities associated with Quercus liaotungensis in different habitats across northern China. Mycorrhiza, 27(5): 441-449.

- White, T.J., Bruns, T., Lee, S. and Taylor, J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics: 315-322. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White. T.J. (Eds.). PCR Protocols: A Guide to Methods and Applications. Academic Press, London, 482p.

- Zamani, S.M., 2014. Identification of ectomycorrhizal fungi associated with oak trees in some forests of Iran and investigation of metabolic and transcriptional profiles in Quercus castaneifolia ectmycorrhizal roots. Ph.D. thesis, College of Agriculture, Tarbiat Modares University, Tehran, 228p (In Persian).

- Zarrinkafsh, M., 1998. Fundamentals of Soil Sciences in Relation to Plant and Environment. Islamic Azad University Press, Tehran, 808p (In Persian).

- Zarrinkafsh, M., 2001. Forestry Soil: Interaction of Soil and Plants Regarding Ecological Factors Ecosystems. Published by Research Institute of Forest and Rangelands, Tehran, 376p (In Persian).